In situ AFM detection of the stiffness of the in situ exposed cell nucleus
In situ AFM detection of the stiffness of the in situ exposed cell nucleus
复制标题
原位 AFM 检测原位暴露细胞核的硬度
DOI：
10.1016/j.bbamcr.2021.118985
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发表时间：
2021
影响因子：
5.1
通讯作者：
Chen Yong
中科院分区：
文献类型：
--
作者：
Wang Kun;Qin Ying;Chen Yong
Biomechanical properties of the cell nucleus play critical roles in cell behaviors and functions. As one important biomechanical property, the stiffness (or Young's modulus) of the cell nucleus has been widely investigated by different techniques including atomic force microscopy (AFM). In most of previous studies, the stiffness of the nuclear region of an intact cell or the stiffness of the isolated nucleus was detected. In this study, we developed a strategy forin situdetecting the stiffness of the cell nucleusviaAFM. The extranuclear components of adherent cells (endothelial cells) werein situremoved by Triton X-100 treatment and the bare, adherent nuclei were exposed forin situAFM force measurement. We found that the nuclear regions of intact cells (5.59 ± 1.55 kPa) had a relatively higher average Young's modulus than the nonnuclear regions (1.47 ± 0.77 kPa) and that thein situexposed nuclei (22.06 ± 7.29 kPa) were much stiffer than the nuclear regions of intact cells. This strategy is very simple and effective for detecting the stiffness of the cell nucleus and potentially is promising for a wide application.
