Persistent infection with small colony variant strains of Staphylococcus aureus in patients with cystic fibrosis
Persistent infection with small colony variant strains of Staphylococcus aureus in patients with cystic fibrosis
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DOI：
10.1086/515238
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发表时间：
1998-04-01
影响因子：
6.4
通讯作者：
Peters, G
中科院分区：
文献类型：
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作者：
Kahl, B;Herrmann, M;Peters, G
在一项为期34个月的前瞻性研究中，确定囊性纤维化(CF)患者中金黄色葡萄球菌小菌落变异(SCV)的患病率，78名患者中有26名患者发现了金黄色葡萄球菌SCV或SCV+正常金黄色葡萄球菌；27名患者仅携带正常金黄色葡萄球菌。用脉冲凝胶电泳法对同一时间分离的SCV和正常株，以及连续采集的病人标本中的多株金黄色葡萄球菌SCV和正常株进行了克隆性鉴定。所有的金黄色葡萄球菌SCV都对抗叶酸抗生素耐药，而相应的亲本株是敏感的，在12对SCV/正常株中的11对中，庆大霉素对SCV表型的金黄色葡萄球菌的活性低于对正常分离株。对SCV的潜在营养缺乏性分析揭示了氯化血红素、胸腺嘧啶核苷和/或甲萘二酮的依赖，因此，金黄色葡萄球菌SCV在CF患者的呼吸道分泌物中高度流行，持续时间较长，可能是导致CF患者金黄色葡萄球菌持续存在的原因。
In a 34-month prospective study to determine the prevalence of Staphylococcus aureus small colony variants (SCVs) in cystic fibrosis (CF) patients, S. aureus SCVs or SCVs plus normal S. aureus were recovered from 26 of 78 patients; 27 patients harbored only normal S. aureus. By pulsed-held gel electrophoresis, clonal identity was demonstrated of SCV and normal strains isolated at the same time and of multiple S. aureus SCV and normal strains in consecutive specimens from individual patients. All S. aureus SCVs were resistant to antifolate antibiotics, while the corresponding parent strains were susceptible, and in 11 of 12 SCV/normal pairs, gentamicin was less active against S. aureus with the SCV phenotype than against the normal isolate, Analysis of the underlying auxotrophism of SCVs revealed hemin, thymidine, and/or menadione dependencies, Thus, S. aureus SCVs are highly prevalent in respiratory secretions of CF patients, persist over extended periods, and may contribute to S. aureus persistence in CF patients.