PLD alpha 1 and GPA1 are involved in the stomatal closure induced by Oridonin in Arabidopsis thaliana
PLD alpha 1 and GPA1 are involved in the stomatal closure induced by Oridonin in Arabidopsis thaliana
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PLD α 1 和 GPA1 参与拟南芥冬凌草甲素诱导的气孔关闭
DOI：
10.1071/fp21156
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发表时间：
2021
影响因子：
3
通讯作者：
Ning Yang
中科院分区：
文献类型：
--
作者：
Yue Zhang;Ruirui Liu;Yaping Zhou;Simin Wang;Bianfeng Zhang;Juantao Kong;Sheng Zheng;Ning Yang
冬凌草甲素是一种重要的二萜类化合物，在植物生长发育中发挥着重要作用。PLDα1和GPA1参与了许多生物或非生物胁迫。本研究以拟南芥野生型(WT)、pldα1缺陷突变体(pldα1)、gpa1缺陷突变体(Gpa1)和pldα1/gpa1双突变体为材料，研究了冬凌草甲素对气孔开度的影响以及pldα1和gpa1在这一反应中的作用。结果表明，60μ·L-1冬凌草甲素可诱导气孔关闭，并显著增加GPA1和PLDα1的相对表达水平。冬凌草甲素通过抑制抗氧化酶而增加保卫细胞中H_2O_2的积累。过氧化氢增加导致气孔关闭正向调控基因OST1的表达。冬凌草甲素诱导的气孔关闭过程中，PLDα1和GPA1均起作用，PLDα1在GPA1下游起作用。结果表明，冬凌草甲素通过影响GPA_1，促进PLDα_1产生PA，进而积累H_2O_2上调OST_1基因，从而导致气孔关闭。
Oridonin is an important diterpenoid, which plays an important role in plant growth and development. PLDα1 and GPA1 are involved in many biotic or abiotic stresses. In this study, using the seedlings of Arabidopsis thaliana L. wild type (WT), PLDα1 defective mutant (pldα1), GPA1 defective mutant (gpa1) and pldα1/gpa1 double mutant as materials, the effect of stomatal apertures responding to Oridonin and the functions of PLDα1 and GPA1 in this response were investigated. The results showed that 60 μmol·L–1 of Oridonin induced stomatal closure and significantly increased the relative expression levels of GPA1 and PLDα1. Oridonin increased H2O2 accumulation in guard cells by inhibiting the antioxidant enzymes. The increase of H2O2 caused the expression of OST1, which is a positive regulatory gene for stomatal closure. Both PLDα1 and GPA1 were involved in Oridonin-induced stomatal closure and PLDα1 acted downstream of GPA1. The results suggested that Oridonin caused stomatal closure by affecting GPA1 and promoting PLDα1 to produce PA, and further accumulating H2O2 to upregulate gene OST1.