Na entry and Na‐K pump activity in murine, hamster, and human cells ‐ effect of monensin, serum, platelet extract, and viral transformation
Na entry and Na‐K pump activity in murine, hamster, and human cells ‐ effect of monensin, serum, platelet extract, and viral transformation
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小鼠、仓鼠和人类细胞中的钠进入和钠钾泵活性 - 莫能菌素、血清、血小板提取物和病毒转化的影响
DOI：
10.1002/jcp.1041030104
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发表时间：
1980
影响因子：
5.6
通讯作者：
E. Rozengurt
中科院分区：
文献类型：
--
作者：
S. Mendoza;N. Wigglesworth;P. Pohjanpelto;E. Rozengurt
在不同类型的细胞中，通过检测奈诺莫纳菌素的作用、血清促有丝分裂作用以及SV40和多瘤病毒的致癌作用，研究了Na进入与Na-K泵活性的关系。我们发现，在静止培养的小鼠、仓鼠和人类细胞中，莫能菌素的添加增加了细胞内的钠。在每种情况下，莫能菌素对细胞内Na的升高都与Na-K泵活性的增加有关，Na-K泵的活性被测量为哇巴因可抑制86Rb的摄取。在静止培养中加入血清可刺激所有研究类型的细胞摄取86Rb。血清单独引起细胞内钾的增加，而细胞内钠的变化不一致。在Na-K泵抑制剂哇巴因存在的情况下，血清引起细胞内Na显著增加，而细胞内K几乎没有变化。这种模式被解释为血清的主要作用是增加Na进入细胞内。低浓度莫能菌素(0.2μg/ml)对离子通量和离子含量的影响与血清相似，这支持了莫能菌素和莫能菌素以相同的方式刺激86Rb摄取的结论，即通过增加钠进入细胞内。此外，部分纯化的血小板提取物可刺激静止的3T3细胞的钠进入和86Rb摄取。最后，经SV40或多瘤病毒转化的3T3细胞表现出比未转化的3T3细胞更高的Na内入率和Na-K泵活性。这些结果表明，Na内流速度在调节Na-K泵的活性中起着重要作用，Na和K运动的增加是血清在多种细胞类型中引起的快速反应。
The relationship between Na entry and the activity of the Na‐K pump has been investigated in a variety of cell types by testing the effect of the Naionophore monensin, mitogenic stimulation with serum and oncogenic transformation by SV40 and polyoma virus. We found that addition of monensin increases intracellular Na in quiescent cultures of murine, hamster, and human cells. In each case, the rise in intracellular Na by monensin is associated with an increase in the activity of the Na‐K pump, which was measured as ouabain‐inhibitable 86Rb uptake. The addition of serum to quiescent cultures stimulates 86Rb uptake in all cell types studied. Serum alone causes an increase in intracellular potassium with no consistent change in intracellular Na. In the presence of the Na‐K pump inhibitor ouabain, serum causes a marked increase in intracellular Na, with little change in intracellular K. This pattern is interpreted as indicating that the primary effect of serum is to increase Na entry into the cells. A low concentration of monensin (0.2 μg/ml) mimics the effect of serum on ion fluxes and content, which supports the conclusion that serum and monensin stimulate 86Rb uptake in the same manner, namely by increasing Na entry into the cells. In addition, a partially purified platelet extract stimulates Na entry and 86Rb uptake in quiescent 3T3 cells. Finally 3T3 cells transformed by SV40 or polyoma virus exhibit a higher rate of Na entry and of Na‐K pump activity than their untransformed 3T3 counterparts. All these results indicate that the rate of Na entry plays an important role in the regulation of the activity of the Na‐K pump and that an increase in Na and K movements is a rapid response elicited by serum in a variety of cell types.
DOI：
10.1002/9780470720974.ch7
发表时间：
1985
期刊：
Ciba Foundation symposium
影响因子：
--
作者：
R. Ross;D. Bowen-Pope;E. Raines
通讯作者：
R. Ross;D. Bowen-Pope;E. Raines