The SUR2 gene of Arabidopsis thaliana encodes the cytochrome P450CYP83B1, a modulator of auxin homeostasis
The SUR2 gene of Arabidopsis thaliana encodes the cytochrome P450CYP83B1, a modulator of auxin homeostasis
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DOI：
10.1073/pnas.260502697
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发表时间：
2000-12-19
影响因子：
11.1
通讯作者：
Bellini, C
中科院分区：
文献类型：
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作者：
Barlier, I;Kowalczyk, M;Bellini, C
已经进行了遗传筛选以鉴定拟南芥中具有改变的生长素稳态的突变体。在转座子诱变的群体中鉴定了生长素过量产生突变体sur2的标记等位基因。SUR2基因被克隆并显示编码CYP83B1蛋白，该蛋白属于P450依赖性单加氧酶的targe家族。SUR2的表达上调sur1突变体和诱导外源生长素在野生型。对sur2植物中吲哚乙酸（IAA）合成和代谢的分析表明，该突变通过上调IAA合成而导致IAA池大小的条件性增加。
Genetic screens have been performed to identify mutants with altered auxin homeostasis in Arabidopsis. A tagged allele of the auxin-overproducing mutant sur2 was identified within a transposon mutagenized population. The SUR2 gene was cloned and shown to encode the CYP83B1 protein, which belongs to the targe family of the P450-dependent monooxygenases. SUR2 expression is up-regulated in sur1 mutants and induced by exogenous auxin in the wild type. Analysis of indole3-acetic acid (IAA) synthesis and metabolism in sur2 plants indicates that the mutation causes a conditional increase in the pool size of IAA through up-regulation of IAA synthesis.