Enhanced levels of soluble and membrane-bound CD137 levels in patients with acute coronary syndromes
Enhanced levels of soluble and membrane-bound CD137 levels in patients with acute coronary syndromes
复制标题
DOI：
10.1016/j.cca.2009.12.011
复制
发表时间：
2010-03-02
影响因子：
5
通讯作者：
Ping, Yang
中科院分区：
文献类型：
--
作者：
Dongming, Liu;Zuxun, Lu;Ping, Yang
背景资料：越来越多的证据表明，肿瘤坏死因子超家族的共刺激分子如CD 40/CD 40配体和OX 40/OX 40配体与动脉粥样硬化有关。为探讨肿瘤坏死因子超家族成员CD 137在急性冠状动脉综合征（ACS）中的表达水平及其与ACS的相关性，选择210例ACS患者（包括稳定型心绞痛（SA）70例，不稳定型心绞痛（UA）80例，急性心肌梗死（AMI）60例），并与30例正常人进行对照。流式细胞术检测外周血单核细胞CD 137的表达。血清可溶性CD 137（sCD 137）和C反应蛋白水平通过市售ELISA测定。UA [14.2 ± 3.5平均荧光强度（MFI）]和AMI患者外周血单核细胞中CD 137的表达（15.1 +/- 4.4 MFI）显著高于SA住院患者（6.5 +/- 2.4 MFI）和对照组（7.1 +/- 3.5 MFI）。UA和AMI患者sCD 137分别为16.7 ± 4.9 ng/ml和19.1 ± 43 ng/ml，显著高于SA和对照组sCD 137分别为3.4 ± 1.4 ng/ml和3.9 ± 1.3 ng/ml（p < 0.01）。
Background: Increasing evidence shows that costimulatory molecules of the tumor necrosis factor superfamily such as CD40/CD40 ligand and OX40/OX40 ligand have been implicated in atherosclerosis. We investigated whether the expression levels of the tumor necrosis factor superfamily members CD137 in serum and membrane-bound were related to acute coronary syndromes (ACS).Methods: Thirty normal controls and 210 patients, including 70 with stable angina (SA), 80 with unstable angina (UA), and 60 with acute myocardial infarction (AMI), were enrolled in our study. The expression of CD137 in peripheral monocytes was analyzed by flow cytometry. Serum soluble CD137 (sCD137) and C-reactive protein levels were measured by commercially available ELISA.Results: The expression of CD137 in peripheral monocytes in patients with UA [14.2 +/- 3.5 mean fluorescence intensity (MFI)] and AMI (15.1 +/- 4.4 MFI) was significantly higher than those inpatients with SA (6.5 +/- 2.4 MFI) and controls (7.1 +/- 3.5 MFI). sCD137 in patients with UA (16.7 +/- 4.9 ng/ml) and AMI (19.1 +/- 43 ng/ml) were significantly higher than those in patients with SA (3.4 +/- 1.4 ng/ml) and controls (3.9 +/- 1.3 ng/ml) (p