Genetic variation and evolution of the alpine bamboos (Poaceae: Bambusoideae) using DNA sequence data
Genetic variation and evolution of the alpine bamboos (Poaceae: Bambusoideae) using DNA sequence data
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DOI：
10.1007/pl00013993
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发表时间：
2001-09-01
影响因子：
2.8
通讯作者：
Yang, JB
中科院分区：
文献类型：
--
作者：
Guo, ZH;Chen, YY;Yang, JB
由于木本竹独特的生命周期、形态特征的缺乏甚至分子证据的缺乏，系统发育重建的困难在木本竹中很常见。人们对竹子的遗传变异和种群结构也知之甚少，这阻碍了它们通过育种的利用。本文利用 ITS 序列数据来检查高山竹内的遗传变异程度并探索其系统发育。研究了高山竹子 Thamnocalamus、Fargesia 和 Yushania 三个属的 23 种，以及作为外群的 Ampelocalamus 种。结果表明，白鹤芋 var. crassinodus 和 Fargesia spathacea 分支形成基础类群，但 bootstrap 支持较弱。在其余物种中，包括以前放置在Fargesia（加上Borinda）和Yushania的物种中，F. yunnanensis亚支和F. communis亚支得到了认可，但对这些群体的内部支持仍然很低。结果表明，从形态特征上划分，Fargesia和Yushania在ITS系统发育中不是单系的，需要进一步解析。我们揭示了高山竹子中相对较高水平的遗传变异性，并表明 ITS 区域可用于改善一般木本竹子的通用界定。
Difficulties in phylogenetic reconstruction are common within the woody bamboos due to their unique life cycles, a lack of morphological characters and even an absence of molecular evidence. The genetic variability and population structure of the bamboos are also poorly understood which impedes their exploitation via breeding. In this paper, ITS sequence data were used to examine the degree of genetic variation within the alpine bamboos and to explore their phylogeny. Twenty-three species representing three genera, Thamnocalamus, Fargesia and Yushania, of the alpine bamboos and one species of Ampelocalamus as an outgroup were studied. The results indicated that Thamnocalamus spathiflorus var. crassinodus and the Fargesia spathacea clade form the basal groups but bootstrap support was weak. Among the rest of the species, including species previously placed in Fargesia (plus Borinda) and Yushania, the F. yunnanensis subclade and the F. communis subclade were recognized but internal support for such groups was again low. The result indicated that, Fargesia and Yushania as delimited by morphological characters, are not monophyletic in the ITS phylogeny and require further resolution. We revealed relatively high levels of genetic variability in the alpine bamboos and showed that the ITS region could be used to improve generic delimitation of the woody bamboos in general.