Buckyballs meet viral nanoparticles: candidates for biomedicine.
Buckyballs meet viral nanoparticles: candidates for biomedicine.
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DOI：
10.1021/ja902293w
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发表时间：
2009-12-02
影响因子：
15
通讯作者：
Manchester M
中科院分区：
文献类型：
--
作者：
Steinmetz NF;Hong V;Spoerke ED;Lu P;Breitenkamp K;Finn MG;Manchester M
富勒烯如C60在几种新兴技术中显示出作为功能组分的前景。对于生物医学应用，C60已用于基因和药物递送载体、成像剂以及癌症治疗中的光敏剂。C60用于生物应用的主要缺点是其在水中的不溶性。为了克服这一限制，我们将C60衍生物共价连接到豇豆花叶病毒和噬菌体Qβ病毒样颗粒上，作为天然存在的病毒纳米颗粒（VNP）结构的例子，这些结构已被证明是生物医学的有前途的候选物。采用两种不同的标记策略，产生水溶性和稳定的VNP-C60和VNP-PEG-C60缀合物。使用透射电子显微镜、扫描透射电子显微镜（STEM）、凝胶电泳、分子排阻色谱、动态光散射和蛋白质印迹法对样品进行表征。使用PEG修饰的炔丙基-O-PEG-C60衍生物的“点击”化学生物缀合在VNP表面产生了高负载的富勒烯，通过STEM对各个C60单元的成像表明了这一点。通过共聚焦显微镜发现染料标记的VNP-PEG-C60复合物在人癌细胞系中的细胞摄取是稳健的，表明细胞内化不被所附的C60单位抑制。这些结果为开发具有潜在应用前景的新型治疗设备打开了大门。
Fullerenes such as C60 show promise as functional components in several emerging technologies. For biomedical applications, C60 has been used in gene- and drug-delivery vectors, as imaging agents, and as photosensitizers in cancer therapy. A major drawback of C60 for bioapplications is its insolubility in water. To overcome this limitation, we covalently attached C60 derivatives to Cowpea mosaic virus and bacteriophage Qβ virus-like particles, as examples of naturally occurring viral nanoparticle (VNP) structures that have been shown to be promising candidates for biomedicine. Two different labeling strategies were employed, giving rise to water-soluble and stable VNP-C60 and VNP-PEG-C60 conjugates. Samples were characterized using a combination of transmission electron microscopy, scanning transmission electron microscopy (STEM), gel electrophoresis, size-exclusion chromatography, dynamic light scattering, and western blotting. “Click” chemistry bioconjugation using a PEG-modified propargyl-O-PEG-C60 derivative gave rise to high loadings of fullerene on the VNP surface, indicated by the imaging of individual C60 units by STEM. The cellular uptake of dye-labeled VNP-PEG-C60 complexes in a human cancer cell line was found by confocal microscopy to be robust, showing that cell internalization was not inhibited by the attached C60 units. These results open the door for the development of novel therapeutic devices with potential applications in photo-activated tumor therapy.
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影响因子：
4.9
作者：
Sitharaman B;Zakharian TY;Saraf A;Misra P;Ashcroft J;Pan S;Pham QP;Mikos AG;Wilson LJ;Engler DA
通讯作者：
Engler DA
影响因子：
15
作者：
Wang, Q;Chan, TR;Finn, MG
通讯作者：
Finn, MG
影响因子：
3.6
作者：
Tornoe, CW;Christensen, C;Meldal, M
通讯作者：
Meldal, M
影响因子：
4.7
作者：
Sen Gupta, S;Kuzelka, J;Finn, MG
通讯作者：
Finn, MG
影响因子：
3.2
作者：
Bruckman, Michael A.;Kaur, Gagandeep;Wang, Qian
通讯作者：
Wang, Qian