Nitric oxide control of drinking, vasopressin and oxytocin release and blood pressure in dehydrated rats.
Nitric oxide control of drinking, vasopressin and oxytocin release and blood pressure in dehydrated rats.
复制标题
一氧化氮控制脱水大鼠的饮酒、加压素和催产素的释放以及血压。
DOI：
10.1016/s0031-9384(97)00528-3
复制
发表时间：
1998
影响因子：
2.9
通讯作者：
Kadekaro,M
中科院分区：
文献类型：
--
作者：
Liu,H;Terrell,ML;Bui,V;Summy-Long,JY;Kadekaro,M
脑室内(i.c.v.)脑室注射一氧化氮合酶抑制剂L(250μg/5μL)可明显减轻缺水24 h大鼠的饮水反应，2 m in后血浆催产素(OT)水平升高，120 m in时催产素和加压素水平均升高。在允许喝水的脱水动物中，没有观察到L对这两种激素的延迟影响。在不允许饮水的脱水大鼠中，注射人工脑脊液(ACSF)后，血压保持稳定。然而，在获得水的大鼠中，有即刻但短暂的升压反应(0-5分钟)，并有45-120分钟的延迟性低血压。无论动物是否饮酒，L-NAME均以双相模式持续升高血压，早期峰值在5分钟，15-30分钟后衰退，第二次升压反应开始于30-45分钟，实验结束后120分钟持续升高。这些升压反应不依赖于肾上腺。因此，中枢产生的一氧化氮有助于饮酒，抑制大细胞系统释放加压素和催产素，并维持正常水分和脱水大鼠的静息动脉血压。
Intracerebroventricular (i.c.v.) injection of the inhibitor of NO synthase (NOS), NG-nitro-l-arginine methyl ester (l-NAME) (250 μg/5 μL) attenuated the drinking response in rats deprived of water for 24 h. Moreover, oxytocin (OT) levels in plasma increased after 2 min, whereas both oxytocin and vasopressin levels were elevated at 120 min after intracerebroventricular injection. The delayed effect of l-NAME on both hormones was not observed in dehydrated animals allowed to drink water. Blood pressure remained stable after injection of artificial cerebrospinal fluid (aCSF) in dehydrated rats not allowed to drink. In rats having access to water, however, there was an immediate but transient pressor response (0–5 min) with a delayed hypotension from 45 to 120 min. l-NAME consistently increased blood pressure in a biphasic mode, whether the animals drank or not, with an early peak at 5 min that decayed after 15–30 min and a second pressor response beginning at 30–45 min and remaining elevated at 120 min when the experiment ended. These pressor responses were independent of the adrenal glands. Thus, centrally produced nitric oxide facilitates drinking, inhibits release of vasopressin and oxytocin from the magnocellular system, and maintains resting arterial blood pressure in normally hydrated and dehydrated rats.