Salicylic acid determination in cow urine and drugs using a bienzymatic sensor.
Salicylic acid determination in cow urine and drugs using a bienzymatic sensor.
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DOI：
10.1016/j.jpba.2006.03.020
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发表时间：
2006-09
影响因子：
3.4
通讯作者：
L. Campanella;E. Gregori;M. Tomassetti
中科院分区：
文献类型：
--
作者：
L. Campanella;E. Gregori;M. Tomassetti
利用Clark型气体扩散电极和包埋在三醋酸纤维素膜中的两种酶（酪氨酸酶和水杨酸羟化酶），研制了一种测定水杨酸（水杨酸根离子）的酶生物传感器。经优化后，将该方法应用于牛尿中水杨酸的测定。使用校准曲线，实现了相对良好的回收率，约为83%-109%，精密度（R.S.D.）可接受。约8%）。目前正在测试该方法，用于测定市售药品或盖伦产品中所含的水杨酸。到目前为止，已发现与标称值的一致性在75%和110%之间，R.S.D.低于8%。
An enzymatic biosensor was developed for salicylic acid (salicylate ion) determined using a Clark type gas diffusion electrode and two enzymes (tyrosinase and salicylate hydroxylase) entrapped in a cellulose triacetate membrane. After optimization, the method was applied to the determination of salicylic acid in cow urine. Relatively good recoveries were achieved, between about 83% and 109%, using the calibration curve, and acceptable precision (R.S.D. about 8%). The method is now being tested for the determination of salicylic acid contained in commercially available drug specialities or galenic products. So far agreement with nominal values has been found to be between 75% and 110% with a R.S.D. of less than 8%.