The Structure and Function of Type III Secretion Systems.
The Structure and Function of Type III Secretion Systems.
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DOI：
10.1128/microbiolspec.vmbf-0004-2015
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发表时间：
2016-02
影响因子：
3.7
通讯作者：
Stebbins CE
中科院分区：
文献类型：
--
作者：
Notti RQ;Stebbins CE
III 型分泌系统 (T3SS) 为革兰氏阴性细菌提供了一种改变真核宿主生物学的最亲密的方法——将效应蛋白从细菌细胞质直接传递到真核生物细胞质。这一令人难以置信的生物物理壮举是通过纳米注射器“注射体”实现的，它形成了穿过三个质膜、肽聚糖层和细胞外空间的管道，形成了蛋白质从细菌直接输送到宿主的屏障。本章重点是结构层面的T3SS功能；我们将总结影响我们对这些系统的结构和功能的理解的核心发现，并重点介绍该领域的最新发展。反过来，我们描述了 T3SS 分泌装置，考虑其与分泌底物的结合，并讨论分泌功能的翻译后调节。最后，我们以讨论 T3SS 中结构-功能关系的未来前景作为结束语。
Type III secretion systems (T3SS) afford gram-negative bacteria a most intimate means of altering the biology of their eukaryotic hosts — the direct delivery of effector proteins from the bacterial cytoplasm to that of the eukaryote. This incredible biophysical feat is accomplished by nanosyringe “injectisomes,” which form a conduit across the three plasma membranes, peptidoglycan layer and extracellular space that form a barrier to the direct delivery of proteins from bacterium to host. The focus of this chapter is T3SS function at the structural level; we will summarize the core findings that have shaped our understanding of the structure and function of these systems and highlight recent developments in the field. In turn, we describe the T3SS secretory apparatus, consider its engagement with secretion substrates, and discuss the post-translational regulation of secretory function. Lastly, we close with a discussion of the future prospects for the interrogation of structure-function relationships in the T3SS.