Nitroxide-radicals-modified gold nanorods for in vivo CT/MRI-guided photothermal cancer therapy.
Nitroxide-radicals-modified gold nanorods for in vivo CT/MRI-guided photothermal cancer therapy.
复制标题
用于体内 CT/MRI 引导光热癌症治疗的氮氧自由基修饰金纳米棒
DOI：
10.2147/ijn.s171804
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发表时间：
2018
影响因子：
8
通讯作者：
Hu Y
中科院分区：
文献类型：
--
作者：
Xia L;Zhang C;Li M;Wang K;Wang Y;Xu P;Hu Y
本文介绍了氮氧自由基修饰的金纳米棒（Au-TEMPO NRs）用于成像引导光热癌症治疗的合成，表征和生物医学应用的报告。采用晶种法合成了Au纳米棒，并加入4-Amino-TEMPO，在磁力搅拌下进行反应。Au-TEMPO纳米棒的平均长度为39.2 nm，平均长径比约为3.85，在808 nm激光照射下表现出良好的光热性能。Au-TEMPO NRs在PBS中可保存1个月以上，在浓度高达3 mg/mL时对肿瘤细胞和正常细胞均无细胞毒性，由于其对Au的高X射线衰减和对氮氧自由基的良好r1弛豫能力，可作为体内外CT/MR成像的双模式造影剂。此外，它们在主要器官中的保留时间较长（约4小时），这使得诊断的CT/MR成像时间窗较长。生物分布结果显示，这些Au-TEMPO NR被动聚集在肝脏和脾脏。在808 nm激光照射下，Au-TEMPO NRs可消融4 T1荷瘤小鼠的实体瘤，有望成为一种新型的双模式成像和光热治疗肿瘤的治疗诊断剂。这种具有CT/MR成像和光热治疗能力的Au-TEMPO纳米棒在影像引导的光热肿瘤治疗中具有积极的作用。
This article presents a report of the synthesis, characterization, and biomedical application of nitroxide-radicals–modified gold nanorods (Au-TEMPO NRs) for imaging-guided photothermal cancer therapy. Au nanorods were synthesized through seed-mediated growth method, 4-Amino-TEMPO was added and the reaction proceeded under magnetic stirring. With a mean length of 39.2 nm and an average aspect ratio of approximately 3.85, Au-TEMPO NRs showed good photothermal ability when they were irradiated by 808-nm laser. Au-TEMPO NRs could be stored in PBS for more than 1 month, showed no cytotoxicity against both tumor and normal cells at a concentration of up to 3 mg/mL, and functioned as a dual-mode contrast agent for CT/magnetic resonance (MR) imaging in vitro and in vivo, due to their high X-ray attenuation of Au and good r1 relaxivity of nitroxide radicals. Further, they had a long retention time (~4 hours) in the main organs, which enabled a long CT/MR imaging time window for diagnosis. Bio-distribution results revealed that these Au-TEMPO NRs passively aggregated in the liver and spleen. After irradiation by 808-nm laser, Au-TEMPO NRs could ablate the solid tumor in 4T1 tumor-bearing mice, which implied they were a potential theranostic agent for dual-mode imaging and photothermal cancer therapy. This type of Au-TEMPO NRs with the abilities of CT/MR imaging and photothermal therapy, can play an active role in imaging-guided photothermal cancer therapy.