Effect of copper exposure on reproductive ability in the bank vole (Myodes glareolus)
Effect of copper exposure on reproductive ability in the bank vole (Myodes glareolus)
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DOI：
10.1007/s10646-014-1295-6
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发表时间：
2014-08
期刊：
影响因子：
2.7
通讯作者：
Agata Miska-Schramm;M. Kruczek;J. Kapusta
中科院分区：
文献类型：
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作者：
Agata Miska-Schramm;M. Kruczek;J. Kapusta
由于人类活动，自然生态系统中的铜含量正在稳步增加。它在植物中积累，对食草动物构成威胁。在污染地区，观察到小型啮齿动物的种群密度较低。啮齿动物数量的下降可能是由于死亡率增加或生育力下降造成的。这项研究考察了铜对岸鼠繁殖活动的影响，这是一种小型啮齿动物，在觅食过程中经常漫步到可能受到污染的田野中。这些动物被用0,150或600ppm铜的溶液处理。在暴露12周后，对雄性精子的质量和数量进行了测试。为了评估形态发育，我们比较了试验组的体重，雄性睾丸和附性腺的重量，雌性的子宫，以及受试雌性的成熟卵巢卵泡的数量。在这两种剂量下，铜都会导致精子数量减少，并导致精子头部畸形。较高剂量影响精子尾膜的完整性、存活率和活力。未观察到铜对雄性睾丸形态发育的影响，只有较低剂量的铜增加了睾丸重量。在女性中，高剂量对形态发育有负面影响，而低剂量则增加子宫重量。铜对卵泡数无明显影响。首次报道了岸田鼠最典型的卵泡形态。
The amount of copper in natural ecosystems is steadily increasing, due to human activities. It accumulates in plants, posing a threat to herbivores. In polluted areas the population density of small rodents is observed to be lower. The decline in rodent numbers may be caused by increased mortality or diminished fertility. This study examined the effect of copper on the reproductive activity of the bank vole (Myodes glareolus), a small rodent which during foraging often wanders into fields where it might be exposed to pollution. The animals were treated with solutions of 0, 150 or 600 ppm Cu. After 12 weeks of exposure the quality and quantity of the male’s sperm was tested. To assess morphological development we compared the experimental groups for body weight, the weight of the male’s testes and accessory sex glands, the female’s uterus, and the number of matured ovary follicles in tested females. At both doses, copper administration led to lower sperm count and caused sperm head anomalies. The higher dose compromised sperm tail membrane integrity, viability and motility. No effect of copper on morphological development was observed in males, and only the lower dose increased testes weight. In females the higher dose had a negative effect on morphological development, and the lower dose increased uterus weight. No effect of copper on ovarian follicle number was found. For the first time, the morphology of the most typical ovarian follicles of the bank vole is presented.