Developmental roles of the spontaneous depolarization wave in synaptic network formation in the embryonic brainstem
Developmental roles of the spontaneous depolarization wave in synaptic network formation in the embryonic brainstem
复制标题
自发去极化波在胚胎脑干突触网络形成中的发育作用
DOI：
10.1016/j.neuroscience.2017.09.030
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发表时间：
2017
期刊：
影响因子：
3.3
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Momose-Sato;Y. and Sato;K.
在发育中的中枢神经系统中最早表达的活动之一是广泛传播的波状活动，我们称之为去极化波。尽管对该活动的全球特征有相当大的共识，但其生理作用仍有待澄清。去极化波是在功能性突触发生的特定时期表达的，这一发育特征导致了去极化波在突触网络组织中起一定作用的假设。本研究通过抑制卵形去极化波对鸡胚迷走神经相关脑干核功能突触发育的影响，验证了这一假说。慢性抑制去极化波对迷走神经感觉通路一级核（孤束核和对侧非神经束核）的单突触兴奋性突触后电位的发育时间、振幅和空间分布无显著影响，但降低了高阶核（臂旁核）的多突触反应。这些结果表明，去极化波在脑干功能突触表达的初始过程中起重要作用，特别是在颅感觉通路的高阶核中。
One of the earliest activities expressed within the developing central nervous system is a widely propagating wave-like activity, which we referred to as the depolarization wave. Despite considerable consensus concerning the global features of the activity, its physiological role is yet to be clarified. The depolarization wave is expressed during a specific period of functional synaptogenesis, and this developmental profile has led to the hypothesis that the wave plays some roles in synaptic network organization. In the present study, we tested this hypothesis by inhibiting the depolarization wavein ovoand examining its effects on the development of functional synapses in vagus nerve-related brainstem nuclei of the chick embryo. Chronic inhibition of the depolarization wave had no significant effect on the developmental time course, amplitude, and spatial distribution of monosynaptic excitatory postsynaptic potentials in the first-order nuclei of the vagal sensory pathway (the nucleus of the tractus solitarius (NTS) and the contralateral non-NTS region), but reduced polysynaptic responses in the higher-order nucleus (the parabrachial nucleus). These results suggest that the depolarization wave plays an important role in the initial process of functional synaptic expression in the brainstem, especially in the higher-order nucleus of the cranial sensory pathway.