Complete nucleotide sequence of a California isolate of Radish mosaic virus
Complete nucleotide sequence of a California isolate of Radish mosaic virus
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DOI：
10.1007/s00705-008-0229-0
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发表时间：
2008-10
影响因子：
2.7
通讯作者：
K. Komatsu;Kouji Hatada;M. Hashimoto;J. Ozeki;K. Maejima;S. Kagiwada;Y. Yamaji;S. Namba
中科院分区：
文献类型：
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作者：
K. Komatsu;Kouji Hatada;M. Hashimoto;J. Ozeki;K. Maejima;S. Kagiwada;Y. Yamaji;S. Namba
萝卜花叶病毒（RaMV）是Comoviridae科Comovirus属的成员[9]。豇豆花叶病毒具有两个正单链基因组RNA（RNA 1和RNA 2），其被双核苷酸化成单独的球形病毒体。RaMV主要感染十字花科植物，不像大多数Comovirus属的成员，其主要感染豆科植物。RaMV最初于1939年在美国加州发现[12]，但在欧洲、日本、亚洲和非洲也有报道[4，6，10，11]。最近，我们确定了RaMV日本分离株（RaMV-J）的基因组序列，并表明非豆科感染的豇豆花叶病毒（包括RaMV）与豆科感染的豇豆花叶病毒有较远的亲缘关系[8]。有趣的是，除了RaMV-J和捷克RaMV分离株（RaMV 1）之间的RNA依赖性RNA聚合酶（RdRp）序列比较外，RaMV的遗传变异性尚未进行研究。尽管表现出与RaMV-J相似的宿主范围和寄主学[3]，但缺乏关于RaMV的加州分离物的基因组序列的信息。在这里，我们报告了一个完整的基因组序列的RNA 1和RNA 2的一个。
Radish mosaic virus (RaMV) is a member of the genus Comovirus of the family Comoviridae [9]. Comoviruses have two positive-, single-stranded genomic RNAs (RNA1 and RNA2), which are encapsidated into separate spherical virions. RaMV mainly infects cruciferous plants, unlike most members of the genus Comovirus, which predominantly infect plants of the Leguminosae family. RaMV was originally identified in California, USA, in 1939 [12], but its occurrence has also been reported in Europe, Japan, Asia, and Africa [4, 6, 10, 11]. Recently, we determined the genome sequence of a Japanese isolate of RaMV (RaMV-J) and suggested that non-legume-infecting comoviruses, including RaMV, are distantly related to legume-infecting comoviruses [8]. Interestingly, the genetic variability of RaMV has not been examined except for the RNA-dependent RNA polymerase (RdRp) sequence comparisons between RaMV-J and a Czech RaMV isolate (RaMV1). Despite exhibiting similar host ranges and symptomatology to RaMV-J [3], information on the genome sequence of the California isolate of RaMV is lacking. Here, we report the complete genome sequence of both RNA1 and RNA2 of a