Differentiation-dependent changes in human trophoblast expression of decay-accelerating factor are modulated by 3',5' cyclic adenosine monophosphate.
Differentiation-dependent changes in human trophoblast expression of decay-accelerating factor are modulated by 3',5' cyclic adenosine monophosphate.
复制标题
人滋养层腐烂加速因子表达的分化依赖性变化受 3,5 环单磷酸腺苷调节。
DOI：
10.1016/s1071-5576(96)00061-5
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Coutifaris,C
中科院分区：
文献类型：
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作者：
Zarkadis,IK;Omigbodun,A;Forson,A;Ziolkiewicz,P;Kinoshita,T;Lambris,JD;Coutifaris,C
目的：衰变加速因子（Decay-accelerating factor，DIF）是一种补体调节蛋白，可阻止补体介导的细胞溶解。其在人滋养层细胞中的表达取决于其解剖位置和胎龄。其在滋养层中表达调节的机制尚不清楚。本研究的目的是探讨滋养层细胞分化和环磷酸腺苷（cAMP）的影响，对tumor expression.Methods：人滋养层细胞分离绒毛，培养，收获的总RNA提取和北方分析的时间间隔。还通过免疫细胞化学评估了CD 45的表达。结果：随着细胞的分化，滋养层细胞中cAMP的mRNA表达增加，最先出现的是2.2kb的膜结合型cAMP的转录本。随着分化的进行，1.5 kb的转录本成为主要的mRNA形式。暴露于Rp-cAMP的细胞延迟了这一过程，8-溴-cAMP加速了它。Sp-cAMP选择性上调2.2 kb的mRNA转录。结论：人滋养层细胞中TGF β 1的表达依赖于细胞的分化状态，cAMP是这一过程的细胞内调节因子。这种作用可能是通过在其3′ UT区域的mRNA的替代加工介导的，这反过来又影响mRNA的稳定性。
Objective:Decay-accelerating factor (DAF) is a complement regulatory protein that prevents complement-mediated cell lysis. Its expression in human trophoblasts depends on their anatomic location and gestational age. The mechanisms that govern the regulation of its expression in trophoblasts are not understood. The objective of the present study was to investigate the effects of trophoblast diffrentiation and cyclic adenosine monophosphate (cAMP) on DAF expression.Methods:Human trophoblasts were isolated from chorionic villi, cultured, and harvested at timed intervals for total RNA extraction and Northern analysis. Expression of DAF was also assessed by immunocytochemistry. In some cultures, the trophoblasts were exposed to the cAMP agonists 8-bromo-cAMP and Sp-cAMPs or the antagonist Rp-cAMPs.Results:Expression of DAF mRNA increased as the cells differentiated in culture, with the 2.2-kb transcript of membrane-bound DAF appearing first. As differentiation proceeded, the 1.5-kb transcript became the predominant mRNA form. Exposure of the cells to Rp-cAMPs delayed this process; 8-bromo-cAMP accelerated it. Sp-cAMPs selectively up-regulated the 2.2-kb mRNA transcript. Immunocytochemistry confirmed the effects of differentiation on DAF protein expression.Conclusion:Expression of DAF in human trophoblasts is dependent on the state of cell differentiation, and cAMP is an intracellular modulator of this process. This effect may be mediated through alternative processing of DAF mRNA in its 3′ UT region, which in turn affects mRNA stability.