EFFECTS OF DIFFERENT SERUM SUPPLEMENTS IN MATURATION MEDIUM ON MEIOTIC CYTOPLASMIC MATURATION OF PIG OOCYTES
EFFECTS OF DIFFERENT SERUM SUPPLEMENTS IN MATURATION MEDIUM ON MEIOTIC CYTOPLASMIC MATURATION OF PIG OOCYTES
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DOI：
10.1016/0093-691x(93)90433-6
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发表时间：
1993-04-01
期刊：
影响因子：
2.8
通讯作者：
DAY, BN
中科院分区：
文献类型：
--
作者：
FUNAHASHI, H;DAY, BN
在添加0.4%聚乙烯醇(PVA)、10%胎牛血清(FCS)、10%新生仔猪血清(NPS)、10%猪卵泡液(PFF)或10%猪精液(PSF)的培养液中培养20、30、40和50小时后，观察猪卵母细胞减数分裂和细胞质成熟的时间进程。生发泡破裂(GVBD)在FCS和NPS补充剂之间没有差异。培养20h后，FCS和NPS中GVBD的发生率(分别为54%和52%)高于PVA和PFF培养液中的GVBD发生率(32%和33%)，但在40h和50h时差异无统计学意义。添加PSF后，猪卵母细胞在培养20h后染色体迅速凝聚，但所有GVBD卵母细胞在凝聚生发泡期停止发育。培养20小时后受精的卵母细胞未被精子穿透，而30、40和50小时后受精的所有补充剂组均获得较高的穿透率(87%~100%)和多精受精率(86%~100%)。受精后10~12小时，雄性原核形成率分别为28%和28%，而在PVA和PFF中分别为54%和59%。结果表明，添加血清如FCS和NPS降低了猪卵母细胞形成雄性原核的能力，并进一步表明这种不利影响可能是由于成熟事件的加速进行所致。
The temporal progression of meiotic and cytoplasmic maturation of pig oocytes cultured in a medium supplemented with 0.4% polyvinylalcohol (PVA), 10% fetal calf serum (FCS), 10% newborn piglet serum (NPS), 10% porcine follicular fluid (PFF) or 10% porcine seminal fluid (PSF) was examined after 20, 30, 40 and 50 hours of culture. There were no differences in germinal vesicle breakdown (GVBD) among FCS and NPS supplements. After 20 hours of culture, the frequency for GVBD was higher (P < 0.05) in FCS and NPS (54% and 52%, respectively) than in PVA (32%) and PFF (33%) culture media but were not different at 40 and 50 hours of culture. Supplementation with PSF resulted in a rapid chromosome condensation of pig oocytes after 20 hours culture, but all GVBD oocytes stopped developing at the condensed germinal vesicle stage. Oocytes were not penetrated by spermatozoa when inseminated following 20 hours of culture, while high penetration (87 to 100%) and polyspermy rates (86 to 100%) were consistently obtained in all the supplement groups when inseminated after 30, 40 or 50 hours of culture. Male pronuclear formation rates at 10 to 12 hours after insemination, following a 50-hour culture period in FCS and NPS, were 28 and 28%, respectively, in comparison with 54% in PVA and 59% in PFF. The results indicate that supplementing maturation media with serum such as FCS and NPS reduced the ability of pig oocytes to form a male pronucleus, and further suggest that the detrimental effects may be due to accelerated progression of maturation events.