Immuno-electrophoretic study of the human perilymph.
Immuno-electrophoretic study of the human perilymph.
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人类外淋巴的免疫电泳研究。
DOI：
10.3109/00016486009123124
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发表时间：
1960
影响因子：
1.4
通讯作者：
J. Jeanmaire
中科院分区：
文献类型：
--
作者：
L. Chevance;A. Galli;J. Jeanmaire
作者通过免疫电泳法从正常人外淋巴液中分离出13种不同的蛋白质组分。在Grabar装置的平板上同时使用两种抗血清：一种是由巴黎巴斯德研究所(Grabar&Burtin)常规制备和测试的马抗人血清，另一种是作者在叶化后专门制备和储存的兔抗人脊液血清。将本结果与不同作者报道的不涉及外淋巴液而是人正常脊液的电泳组分的结果进行了比较。似乎不支持外淋巴仅仅是脊髓液的透析液的理论。然而，这样的研究在未来可能会对实验室动物外淋巴的各种实验生理病理状态感兴趣。
The authors have isolated by immuno-electrophoretic study thirteen different protein fractions in the normal human perilymph. Two antisera were used (generally simultaneously) on the plate of the Grabar apparatus: a horse, anti-human serum, routinely prepared and tested by the Pasteur Institute of Paris (Grabar & Burtin), and a rabbit, anti-human, spinalfluid serum, specially prepared and stocked after liophylization by the authors for the present study.The comparison of the present results with those reported by various authors dealing not with the perilymph but with the electrophoretic fractions of the human, normal spinal fluid, does not seem to support the theory stating that the perilymph is a mere dialysate from the spinal fluid.Such studies may nevertheless in the future be of interest in various experimental physio-pathological states of the perilymph in laboratory animals.