Photocrosslinked hyaluronic acid hydrogels: Natural, biodegradable tissue engineering scaffolds
Photocrosslinked hyaluronic acid hydrogels: Natural, biodegradable tissue engineering scaffolds
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DOI：
10.1002/bit.10605
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发表时间：
2003-06-05
影响因子：
3.8
通讯作者：
Schmidt, CE
中科院分区：
文献类型：
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作者：
Leach, JB;Bivens, KA;Schmidt, CE
理想情况下，合理设计的组织工程支架可以促进伤口的自然愈合和再生。因此，我们试图合成一种专门设计用于促进组织修复的仿生水凝胶，并选择透明质酸（HA;也称为透明质酸）作为我们的初始材料。透明质酸是一种天然存在的聚合物，与伤口愈合中涉及的各种细胞过程相关，如血管生成。透明质酸还具有独特的优点：易于生产和改性，亲水性和非粘性，可自然生物降解。我们制备了一系列的甲基丙烯酸缩水甘油酯-HA（GMHA）共轭物，随后进行光聚合，形成交联GMHA水凝胶。实现了一定范围的水凝胶降解速率以及相应的适度范围的材料性质（例如，膨胀，筛目尺寸）。共轭甲基丙烯酸酯基团的量增加对应于交联密度增加和降解速率降低，但对人主动脉内皮细胞细胞相容性和增殖的影响不显著。GMHA水凝胶的大鼠皮下植入物显示出良好的生物相容性，几乎没有炎症反应，并且与纤维蛋白阳性对照相比，植入物边缘的血管化水平相似。因此，这些新型GMHA水凝胶适合于用粘附肽序列（例如，RGD）并用于多种伤口愈合应用。(C)2003 Wiley Periodicals，Inc.
Ideally, rationally designed tissue engineering scaffolds promote natural wound healing and regeneration. Therefore, we sought to synthesize a biomimetic hydrogel specifically designed to promote tissue repair and chose hyaluronic acid (HA; also called hyaluronan) as our initial material. Hyaluronic acid is a naturally occurring polymer associated with various cellular processes involved in wound healing, such as angiogenesis. Hyaluronic acid also presents unique advantages: it is easy to produce and modify, hydrophilic and nonadhesive, and naturally biodegradable. We prepared a range of glycidyl methacrylate-HA (GMHA) conjugates, which were subsequently photopolymerized to form crosslinked GMHA hydrogels. A range of hydrogel degradation rates was achieved as well as a corresponding, modest range of material properties (e.g., swelling, mesh size). Increased amounts of conjugated methacrylate groups corresponded with increased crosslink densities and decreased degradation rates and yet had an insignificant effect on human aortic endothelial cell cytocompatibility and proliferation. Rat subcutaneous implants of the GMHA hydrogels showed good biocompatibility, little inflammatory response, and similar levels of vascularization at the implant edge compared with those of fibrin positive controls. Therefore, these novel GMHA bydrogels are suitable for modification with adhesive peptide sequences (e.g., RGD) and use in a variety of wound-healing applications. (C) 2003 Wiley Periodicals, Inc.