Bombyx mori U-shaped regulates the melanization cascade and immune response via binding with the Lozenge protein
Bombyx mori U-shaped regulates the melanization cascade and immune response via binding with the Lozenge protein
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家蚕 U 形通过与菱形蛋白结合调节黑化级联和免疫反应
DOI：
10.1111/1744-7917.12959
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发表时间：
2021
期刊：
影响因子：
4
通讯作者：
Cui Hongjuan
中科院分区：
文献类型：
--
作者：
Zhang Kui;Tan Juan;Hao Xiangwei;Tang Houyi;Abbas Muhammad Nadeem;Su Jingjing;Su Yongyue;Cui Hongjuan
锌指蛋白是一种重要的转录因子，调节着与多种生理和病理过程相关的基因表达。U形，属于加塔之友（FOG）转录因子，作为辅助因子与加塔转录因子相互作用，在造血中起着至关重要的作用。然而，人们对其在昆虫中的功能知之甚少。在本研究中，从家蚕中鉴定和表征了一个U形cDNA，并研究了其在天然免疫中的潜在作用。预测的家蚕U形氨基酸序列在450-459位含有经典的核定位信号（NLS）基序“GESSPKRRRR”，并且456和478位的精氨酸残基是NLS的关键位点。在B的所有测试组织中检测到U形mRNA。然而，在血细胞中发现了最高水平。在大肠杆菌和金黄色葡萄球菌攻击后，血细胞中的U形mRNA表达水平上调。此外，U形敲除显著减少了黑化过程，并抑制了黑化相关基因的表达，包括PPO 1，PPO 2，PPAE和BAEE。此外，U形与菱形蛋白相互作用，以调节昆虫的先天免疫反应。我们的研究结果表明，U形直接结合到菱形蛋白，以调节家蚕的黑化过程和先天免疫反应。
Zinc finger protein, an important transcription factor, regulates gene expression associated with various physiological and pathological processes. U‐shaped, belong to the Friend of GATA (FOG) transcription factor, plays a crucial role in hematopoiesis by interacting with the GATA transcription factor as a co‐factor. However, little is known about its functions in insects. In the present study, a U‐shaped cDNA was identified and characterized from the silkwormBombyx moriand its potential roles in innate immunity investigated. The predicted silkworm U‐shaped amino acid sequence contained a classical nuclear localization signal (NLS) motif “GESSPKRRRR” at position 450–459, and arginine residues at position 456 and 478 are the critical sites of the NLS. U‐shaped mRNA was detected in all tested tissues of theB. mori; however, the highest levels were found in the hemocytes. U‐shaped mRNA expression levels were upregulated in the hemocyte after theEscherichia coliandStaphylococcus aureuschallenge. Furthermore, U‐shaped knockdown significantly reduced the melanization process and suppressed the expression of melanization‐associated genes, includingPPO1,PPO2,PPAEandBAEE. In addition, U‐shaped interacts with Lozenge protein to regulate the innate immune response of the insect. Our results revealed that U‐shaped binds directly to Lozenge protein to modulate the melanization process and innate immune responses in silkworm.