UCA1 functions as a competing endogenous RNA to suppress epithelial ovarian cancer metastasis
UCA1 functions as a competing endogenous RNA to suppress epithelial ovarian cancer metastasis
复制标题
UCA1作为竞争性内源RNA抑制上皮性卵巢癌转移
DOI：
10.1007/s13277-016-4917-1
复制
发表时间：
2016-08-01
期刊：
影响因子：
--
通讯作者：
Cheng, Wenjun
中科院分区：
文献类型：
--
作者：
Yang, Yijun;Jiang, Yi;Cheng, Wenjun
尿路上皮癌相关蛋白1 （UCA1）是参与许多生物过程的功能性长链非编码rna的一个例子。然而，对于上皮性卵巢癌（EOC）中UCA1表达与转移之间的关系知之甚少。本研究结果证实，UCA1不仅在EOC组织和细胞中异常上调，而且与淋巴结转移状态和FIGO分期相关。此外，单因素和多因素分析显示，UCA1是EOC患者总生存的预后因素。在体外实验中，UCA1的敲低降低了EOC细胞的侵袭和迁移能力。结果表明，UCA1可以通过直接结合miR-485-5p发挥内源性海绵的作用。UCA1的缺失参与了miR-485-5p靶基因基质金属肽酶14 （MMP14）表达下调。总之，我们的研究表明UCA1是一种新的EOC预后生物标志物，在UCA1、miR-485-5p和MMP14在EOC转移中建立了新的联系。
Urothelial cancer associated 1 (UCA1) is an example of functional long noncoding RNAs involved in many biologic processes. However, little is known about the association between UCA1 expression and metastasis in epithelial ovarian cancer (EOC). Findings of this study confirmed that not only UCA1 was aberrantly upregulated in EOC tissues and cells, but also correlated with status of lymph node metastasis and FIGO stage. Furthermore, univariate and multivariate analyses showed that UCA1 was a prognostic factor for overall survival in EOC patients. In vitro, knockdown of UCA1 reduced the invasion and migration ability of EOC cells. The results showed that UCA1 could function as an endogenous sponge by directly binding to miR-485-5p. Depletion of UCA1 was involved in the downregulation of matrix metallopeptidase 14 (MMP14) expression, a target gene of miR-485-5p. In conclusion, our work indicates that UCA1 is a new prognostic biomarker for EOC, establishing a novel connection among UCA1, miR-485-5p, and MMP14 in EOC metastasis.