Nanosensors for single cell mechanical interrogation
Nanosensors for single cell mechanical interrogation
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DOI：
10.1016/j.bios.2021.113086
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发表时间：
2021-02-23
影响因子：
12.6
通讯作者：
Chang, Lingqian
中科院分区：
文献类型：
--
作者：
Hang, Xinxin;He, Shiqi;Chang, Lingqian
许多疾病的发生和发展都伴随着生物力学稳态的破坏，有时甚至是由这种破坏决定的。例如，癌细胞和正常细胞表现出不同的细胞机械力表型，因为癌细胞的增殖和侵袭能力往往与肿瘤中机械力的变化有关。通过单细胞分析，可以检测和分析细胞群体中的力学变化，为癌症的研究开辟了新的维度。用于细胞力学研究的纳米传感器设计是一个跨越细胞生物学、力学和微/纳米技术的跨学科领域。在本教程回顾中，我们将深入了解当前可用的细胞力学分析方法的背景和技术创新。首先，我们讨论了肿瘤发生发展过程中机械变化的机制，说明了机械传感器在癌细胞检测中的可行性。接下来，我们总结了常用的细胞力测量技术，包括单分子力谱技术和弹性底物传感器技术的原理、进展和存在的问题。然后，我们讨论了新型微纳尺度机械传感器及其在单细胞水平生物分析中的应用。最后，对细胞机械传感器存在的问题和发展趋势进行了阐述。
The occurrence and development of many diseases are accompanied and sometimes dictated by the destruction of biomechanical homeostasis. For instance, cancer cells and normal cells show different cellular mechanical forces phenotypes, as the proliferation and invasion ability of cancer cells is often related to the changes in mechanical force in the tumor. With single cell analysis, variations in mechanics within a cell population can be detected and analyzed, opening new dimensions in the study of cancer. Nanosensor design for interrogation of cell mechanics is an interdisciplinary area bridging over cell biology, mechanics, and micro/nanotechnology. In this tutorial review, we give insight into the background and technical innovation of currently available methods for mechanical analysis of cells. First, we discuss the mechanism of mechanical changes in the development and progression of cancer that shows the feasibility of mechanical sensors in cancer cell detection. Next, we summarize the principle, progress, and essential problems of common technologies for cell force measurement, including single molecule force spectroscopy and elastic substrate-sensors. Following that, we discuss novel micro and nano-scale mechanical sensors and their applications in single cell level biological analysis. At last, we elaborate on the remaining issues and trends of the cellular mechanical sensors.