Distribution and diversity of PIF-like transposable elements in the Bambusoideae subfamily
Distribution and diversity of PIF-like transposable elements in the Bambusoideae subfamily
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DOI：
10.1016/j.plantsci.2010.05.012
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发表时间：
2010-09
期刊：
影响因子：
5.2
通讯作者：
Mingbing Zhou;Jiang-Jie Lu;Hao Zhong;Xiangning Liu;D. Tang
中科院分区：
文献类型：
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作者：
Mingbing Zhou;Jiang-Jie Lu;Hao Zhong;Xiangning Liu;D. Tang
PIF样转座因子被归类为PIF/Harbinger超家族的成员，该超家族在许多真核生物中已被表征。PIF类转座因子的完整和部分序列已从数百种植物中分离出来，但只有两个已被确定在竹类植物中。从44种竹类植物中分离到139个基因序列，系统发育分析表明PIF类转座因子在竹亚科中广泛存在，具有多样性和丰富性。利用核核糖体DNA的内转录间隔区（ITS）序列，还建立了竹亚科的分子系统学，并与PIF类转座酶基因的树不一致。这些结果表明，类PIF转座因子可能已参与水平转移事件之间的遗传距离远的竹种，或祖先的多态性已遵循不同的进化和随机损失的类PIF转座因子在不同的竹种。中性测试表明，PIF类转座酶基因编码序列在进化过程中受到负选择，PIF类转座酶基因的内含子在负负选择下中性进化。
PIF-like transposable elements were classified as members of the PIF/Harbinger superfamily that has been characterized in many eukaryotes. Complete and partial sequences of PIF-like transposable elements have been isolated from hundreds of plant species, but only two have been identified in bamboo species. We isolated 139 sequences from 44 bamboo species, and phylogenetic analysis showed that PIF-like transposable elements are widespread, diverse and abundant in the Bambusoideae subfamily. A molecular phylogeny of the Bambusoideae subfamily was also established using the internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA, and was incongruent with the tree derived from PIF-like transposase genes. These results indicate that PIF-like transposable elements may have been involved in horizontal transfer events between phylogenetically distant bamboo species, or that an ancestral polymorphism has been followed by divergent evolution and stochastic loss of PIF-like transposable elements in different bamboo species. Neutralist tests show that PIF-like transposase gene coding sequences were negatively selected during evolution and introns of PIF-like transposase genes evolve neutrally with minus negative selection.