Astrocyte Activation in the Brainstem Evoked by Inflammatory Stimulation of the Masticatory Muscle
Astrocyte Activation in the Brainstem Evoked by Inflammatory Stimulation of the Masticatory Muscle
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咀嚼肌炎症刺激引起的脑干星形胶质细胞激活
DOI：
10.1007/978-1-4939-1429-6_19
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
I. Tamura
中科院分区：
文献类型：
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作者：
S. Kumabe;M. Nakatsuka;Ji;Chizuko Inui;Koichiro Jin;S. Jue;Je;Shin;I. Tamura
目的：为了确定咀嚼肌疼痛障碍的合适治疗，向咬肌提供两种刺激以引起炎症，并确定脑干中星形胶质细胞数量变化的时间过程。材料与方法：将50 μl脂多糖（LPS，2 μg/kg）溶于生理盐水中，注入SD大鼠左咬肌（LMM）。恢复24 h后，实验组注射6%氯化钠溶液50 μl，每90 min注射5次，对照组注射溶于生理盐水的LPS溶液50 μl（2 μg / kg）。在给予试剂后1、3或7天解剖LMM和脑干，并制备冷冻切片。免疫组织化学染色检测咬肌中肿瘤坏死因子α（TNFα）和缓激肽受体B2（BKRB 2）的表达，以及脑干中星形胶质细胞标志物和磷酸化细胞外信号调节激酶（pERK）的表达。结果如下：实验组和对照组LMM中TNFα和BKRB 2的表达在刺激后1d显著增加，但在刺激后7 d基本恢复正常。在三叉神经尾侧亚核（Vc），刺激后7 d，实验组胶质细胞酸性蛋白（GFAP）和pERK免疫反应阳性（IR）细胞数量明显增加。pERK-IR阳性细胞分布于Vc背内侧、背外侧和中央区。结论：当通过给予LPS（其是一种炎症因子）和6%氯化钠溶液（其是一种侵害因子）诱导咀嚼肌中的炎症时，Vc中活化的星形胶质细胞和pERK持续约1周，即使在局部炎症消退之后，这表明向慢性疼痛的转变。
Objective: To identify a suitable treatment for masticatory muscle pain disorder, two stimuli were provided to the masseter muscle to cause inflammation, and the time course of changes in the number of astrocytes in the brainstem was determined. Materials and Methods: A solution of 50 μl of lipopolysaccharide (LPS, 2 μg/kg) dissolved in saline was administered to the left masseter muscle (LMM) of Sprague–Dawley rats. After 24 h of recovery, the experimental group received 50 μl of a 6% sodium chloride solution 5 times every 90 min and the control group received another 50 μl of the LPS solution (2 μg / kg) dissolved in saline. The LMM and brainstem were dissected 1, 3, or 7 days after administering the reagents, and frozen sections were prepared. The expression of tumor necrosis factor α (TNFα) and bradykinin receptor B2 (BKRB2) in the masseter muscle and of an astrocytic marker and phosphorylated extracellular signal-regulated kinase (pERK) in the brainstem was examined by immunohistochemical staining. Results: The expression of TNFα and BKRB2 in the LMM markedly increased 1 day after stimulation in the experimental and control groups, but the levels returned to nearly normal 7 days after stimulation. In the trigeminal subnucleus caudalis (Vc), there was a marked increase in the number of glial fibrillary acidic protein (GFAP) and pERK-immunoreactive (IR) cells 7 days after stimulation in the experimental group. pERK-IR cells were observed in the dorsomedial, laterodorsal and central regions of the Vc. Conclusions: When inflammation in the masticatory muscle was induced by the administration of LPS, which is an inflammatory factor, and a 6% sodium chloride solution, which is an infringing factor, activated astrocytes and pERK in the Vc persisted for around 1 week, even after local inflammation had subsided, which suggests a transition to chronic pain.
肌肉损伤引起的大鼠慢性疼痛样行为的评估。（日语）
DOI：
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发表时间：
2006
期刊：
Journal of the Japan Society of Acupuncture and Moxibustion 56
影响因子：
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作者：
Hashimoto T;Kumazawa T
通讯作者：
Kumazawa T
DOI：
10.1248/yakushi.130.281
发表时间：
2010-03
期刊：
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
影响因子：
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作者：
H. Shirakawa;T. Nakagawa;S. Kaneko
通讯作者：
H. Shirakawa;T. Nakagawa;S. Kaneko
颞下颌关节紊乱和紧张型头痛之间的病理生理学重叠
DOI：
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发表时间：
2017
期刊：
影响因子：
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作者：
佐藤 仁;Hitoshi Sato
通讯作者：
Hitoshi Sato