Purification and characterization of six cytochrome P-450 isozymes from human liver microsomes.
Purification and characterization of six cytochrome P-450 isozymes from human liver microsomes.
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人肝微粒体中六种细胞色素 P-450 同工酶的纯化和表征。
DOI：
10.1021/bi00292a019
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Guengerich,FP
中科院分区：
文献类型：
--
作者：
Wang,PP;Beaune,P;Kaminsky,LS;Dannan,GA;Kadlubar,FF;Larrey,D;Guengerich,FP
Philip P. Wang、Philippe Beaune、Laurence S.卡明斯基Dannan，FredF. Kadlubar，Dominique Larrey，and F. PeterGuengerich* 摘要：从4名人体器官捐献者的肝脏中纯化了6种细胞色素P-450（P-450）同工酶，其中3种同工酶是从单个个体中纯化的。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、肽图、一氧化碳-亚铁复合物光谱分析、双向扩散免疫电泳或交叉免疫电泳技术测定的部分或全部参数，在所有6种P-450之间均存在差异（十二烷基硫酸钠-聚丙烯酰胺凝胶电泳/过氧化物酶偶联染色）与兔抗血清提高到5个P-450，或对d-苄非他明、苯并[a]芘、乙酰苯胺、异喹胍、（J？）-和（S）-华法林和1-萘胺。虽然NADPH强化
Philip P. Wang, Philippe Beaune, Laurence S. Kaminsky, Ghazi A. Dannan, FredF. Kadlubar, Dominique Larrey, and F. PeterGuengerich* abstract: Six cytochrome P-450 (P-450) isozymes were purified to electrophoretic homogeneity from the livers of four human organ donors, with three of these isozymes purified from a single individual. Differences were noted between all six P-450s for some or all of the parameters determined by the techniques of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peptide mapping, spectral analysis of ferrous-carbon monoxide complexes, double-diffusion immunoprecipitin analysis or crossed immunoelectrophoresis (sodium dodecyl sulfate-polyacrylamide gel electrophoresis/peroxidase-coupled staining) with rabbit antisera raised to five of the P-450s, or catalytic activity toward d-benzphetamine, benzo [a] pyrene, acetanilide, debrisoquine,(J?)-and (S)-warfarin, and 1-naphthylamine. While NADPH-fortified