Evaluation of environmental conditions for production of bacteriocin-like substance by Bacillus sp. strain P34
Evaluation of environmental conditions for production of bacteriocin-like substance by Bacillus sp. strain P34
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DOI：
10.1007/s11274-007-9520-6
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发表时间：
2008-05
影响因子：
4.1
通讯作者：
A. Motta;A. Brandelli
中科院分区：
文献类型：
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作者：
A. Motta;A. Brandelli
研究了温度、pH和培养基对芽孢杆菌产细菌素的影响。研究了P34。通过析因设计和响应面法（RSM）评价温度和初始pH值的影响。结果的统计分析表明，在所研究的范围内，这两个变量对细菌素的产生有显着影响。响应面数据显示，在初始pH值介于6.0和8.0之间以及温度介于25和37 °C之间时，产生最大的抗微生物活性。没有观察到细菌生长和细菌素产生之间的关系。响应面法被证明是一个强有力的工具，在优化生产的抗菌活性芽孢杆菌。P34。当不同的媒体进行了测试，最大的细菌素生产中观察到大豆蛋白为基础的培养基，但没有实现的鱼粉，羽毛粉，乳清和葡萄废料培养的抗菌活性。
The influence of temperature, pH and media on bacteriocin production byBacillussp. P34 was investigated. The effect of temperature and initial pH was evaluated by factorial design and response surface methodology (RSM). Statistical analysis of results showed that, in the range studied, the two variables have a significant effect on bacteriocin production. Response-surface data showed maximum antimicrobial activity production at initial pH values between 6.0 and 8.0 and temperatures between 25 and 37 °C. No relationship between bacterial growth and bacteriocin production was observed. RSM proved to be a powerful tool in optimizing the production of antimicrobial activity byBacillussp. P34. When different media were tested, maximum bacteriocin production was observed in soybean protein-based medium, but antimicrobial activity was not achieved by cultivation on fish meal, feather meal, whey and grape waste.