Triptolide simultaneously induces reactive oxygen species, inhibits NF-κB activity and sensitizes 5-fluorouracil in colorectal cancer cell lines
Triptolide simultaneously induces reactive oxygen species, inhibits NF-κB activity and sensitizes 5-fluorouracil in colorectal cancer cell lines
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DOI：
10.1016/j.canlet.2009.10.013
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发表时间：
2010-05-28
期刊：
影响因子：
9.7
通讯作者：
Wang, Weiguang
中科院分区：
文献类型：
--
作者：
Xu, Bing;Guo, Xiaoxia;Wang, Weiguang
研究了雷公藤甲素（PG490）对5-氟尿嘧啶（5-FU）细胞毒性的体外化学增敏作用。PG490对结直肠癌细胞株具有毒性（IC(50)s: 1.39 ~ 5.51 nM）。5-FU与低浓度PG490联合使用后，对结直肠癌细胞株的细胞毒性增敏4-15倍。ci -等线图显示PG490与5-FU具有协同作用。PG490和5-FU处理可激活caspase 3和Bax的表达，抑制Bcl-2的表达。PG490同时诱导活性氧（ROS）的产生，抑制NF-kappa B的转录活性。爱思唯尔爱尔兰有限公司2009年版权所有版权所有。
The in vitro chemosensitizing effect of triptolide (PG490) on the cytotoxicity of 5-fluorouracil (5-FU) was determined in three colorectal cancer (CRC) cell lines. PG490 alone was toxic to CRC cell lines (IC(50)s: 1.39-5.51 nM). The cytotoxicity of 5-FU to CRC cell lines was 4-15-fold sensitized by combining use of low concentration of PG490. CI-isobologram indicated that the effect of PG490 plus 5-FU was synergistic. PG490 and 5-FU treatment induced activated caspase 3 and Bax expression and inhibited Bcl-2 expression. PG490 simultaneously induced generation of reactive oxygen species (ROS) and inhibited NF-kappa B transcriptional activity. Crown Copyright (C) 2009 Published by Elsevier Ireland Ltd. All rights reserved.