Driven DNA transport into an asymmetric nanometer-scale pore
Driven DNA transport into an asymmetric nanometer-scale pore
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DOI：
10.1103/physrevlett.85.3057
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发表时间：
2000-10-02
影响因子：
8.6
通讯作者：
Kasianowicz, JJ
中科院分区：
文献类型：
--
作者：
Henrickson, SE;Misakian, M;Kasianowicz, JJ
为了理解单个DNA分子进入纳米级孔的机制，我们研究了多核苷酸诱导的单个铜-溶血素离子通道的离子电流阻断的浓度和电压依赖性。我们发现，封锁频率是成正比的聚合物浓度，它与所施加的电位呈指数增加，DNA进入孔更容易通过入口，具有较大的前庭。我们还测量了限制孔内的改性聚合物对随机扩散和排斥力的电势的最小值。
To understand the mechanism by which individual DNA molecules enter nanometer-scale pores, we studied the concentration and voltage dependence of polynucleotide-induced ionic-current blockades of a single cu-hemolysin ion channel. We find that the blockade frequency is proportional to the polymer concentration, that it increases exponentially with the applied potential, and that DNA enters the pore more readily through the entrance that has the larger vestibule. We also measure the minimum value of the electrical potential that confines a modified polymer inside the pore against random diffusion and repulsive forces.