HERPES-SIMPLEX TYPE-1 DNA IN HUMAN-BRAIN TISSUE
HERPES-SIMPLEX TYPE-1 DNA IN HUMAN-BRAIN TISSUE
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DOI：
10.1073/pnas.78.10.6461
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
KOPROWSKI, H
中科院分区：
文献类型：
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作者：
FRASER, NW;LAWRENCE, WC;KOPROWSKI, H
1型单纯疱疹病毒（HSV-1）潜伏于人体三叉神经节中。该病毒的重新激活会导致皮肤损伤，偶尔可能会感染其他组织，包括大脑。为了确定没有HSV-1感染临床症状的人脑组织是否含有任何HSV-1痕迹，通过核酸内切酶消化、通过凝胶电泳分离片段和通过与标记的HSV-1 DNA探针杂交来检查来自脑组织的DNA。在使用HSV-1基因组的克隆和病毒体纯化片段的实验中，放射自显影检测到杂交带。在11份人脑DNA样本中，有6份发现了HSV-1 DNA序列。在某些情况下，这些条带对应于完整病毒基因组的预期条带;其他条带仅代表基因组的一部分。在某些情况下，可以发现末端片段，这表明DNA是线性的，非整合的形式。
Herpes simplex virus type 1 (HSV-1) resides latently in the trigeminal ganglia of man. Reactivation of this virus causes skin lesions and may occasionally infect other tissues, including the brain. To determine whether the brain tissue of humans free of clinical signs of HSV-1 infection contains any trace of HSV-1, the DNA from brain tissue was examined by endonuclease digestion, separation of the fragments by gel electrophoresis and by hybridization with labeled HSV-1 DNA probes. Hybrid bands were detected autoradiographically in experiments using cloned and virion-purified fragments of the HSV-1 genome. HSV-1 DNA sequences were found in 6 of 11 human brain DNA samples tested. In some cases, these bands corresponded to the bands expected for the complete viral genome; others contained bands representing only a part of the genome. In some cases, the terminal fragments could be found, suggesting that the DNA was in a linear, nonintegrated form.