Molecular analysis of stem cells and their descendants during cell turnover and regeneration in the planarian Schmidtea mediterranea.
Molecular analysis of stem cells and their descendants during cell turnover and regeneration in the planarian Schmidtea mediterranea.
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DOI：
10.1016/j.stem.2008.07.002
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发表时间：
2008-09-11
期刊：
影响因子：
23.9
通讯作者：
Alvarado, Alejandro Sanchez
中科院分区：
文献类型：
--
作者：
Eisenhoffer, George T.;Kang, Hara;Alvarado, Alejandro Sanchez
在成年浮游生物中，由于生理更替或损伤而失去的细胞的替换是由被称为新生母细胞的干细胞的增殖和分化来维持的。新生细胞的谱系关系以及在分化为合适的细胞类型过程中发生的分子变化还知之甚少。在这里，我们报告了一组在新生细胞及其后代中特异表达的基因的鉴定和特征。我们发现，辐射后表达严重下调的基因在分子上定义了至少三个离散的细胞亚群。在完整和再生动物中同时进行的BrdU标记和原位杂交实验表明，这些细胞亚群与血统有关。我们的数据不仅证明了在组织动态平衡和再生过程中测量和研究成体干细胞在体内种群动态的能力，而且表明在浮游生物中的研究对于广泛地了解成体生物中的干细胞生物学是有用的。
In adult planarians the replacement of cells lost to physiological turnover or injury is sustained by the proliferation and differentiation of stem cells known as neoblasts. Neoblast lineage relationships and the molecular changes that take place during differentiation into the appropriate cell types are poorly understood. Here we report the identification and characterization of a cohort of genes specifically expressed in neoblasts and their descendents. We find that genes with severely downregulated expression after irradiation molecularly define at least three discrete subpopulations of cells. Simultaneous BrdU labeling and in situ hybridization experiments in intact and regenerating animals indicate that these cell subpopulations are related by lineage. Our data demonstrate not only the ability to measure and study the in vivo population dynamics of adult stem cells during tissue homeostasis and regeneration, but also the utility of studies in planarians to broadly inform stem cell biology in adult organisms.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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