Serum Protein KNG1, APOC3, and PON1 as Potential Biomarkers for Yin-Deficiency-Heat Syndrome.
Serum Protein KNG1, APOC3, and PON1 as Potential Biomarkers for Yin-Deficiency-Heat Syndrome.
复制标题
血清蛋白 KNG1、APOC3 和 PON1 作为阴虚热证的潜在生物标志物。
DOI：
10.1155/2016/5176731
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Liu C;Mao L;Ping Z;Jiang T;Wang C;Chen Z;Li Z;Li J
阴虚热证是中医学中描述亚健康状态的一个概念。然而，有几个有效的诊断方法可用于确认YDH综合征。为了探索诊断YDH综合征的新方法，我们应用iTRAQ观察了YDH综合征大鼠血清蛋白质谱，并通过ELISA确定蛋白质水平。通过蛋白质组学实验共鉴定出92个差异表达蛋白（63个上调蛋白和29个下调蛋白），主要涉及补体和凝血级联反应以及葡萄糖代谢途径。YDH综合征大鼠血清中激肽原1（KNG 1）显著升高（p < 0.0001），而载脂蛋白C-Ⅲ（APOC 3，p < 0.005）和对氧磷酶1（PON 1，p < 0.001）显著降低。KNG 1、APOC 3和PON 1的组合构成了灵敏度为100.0%、特异性为85.0%的诊断模型。结果表明，KNG 1、APOC 3和PON 1可能作为诊断YDH综合征的潜在生物标志物。KNG 1可能调节YDH综合征中细胞因子和趋化因子的释放，而PON 1和APOC 3的低水平可能分别增加YDH综合征中的氧化应激和脂解。本研究为YDH综合征的诊断提供了一种新的方法，也为深入了解YDH综合征的分子机制提供了有价值的实验依据。
Yin-deficiency-heat (YDH) syndrome is a concept in Traditional Chinese Medicine (TCM) for describing subhealth status. However, there are few efficient diagnostic methods available for confirming YDH syndrome. To explore the novel method for diagnosing YDH syndrome, we applied iTRAQ to observe the serum protein profiles in YDH syndrome rats and confirmed protein levels by ELISA. A total of 92 differentially expressed proteins (63 upregulated proteins and 29 downregulated proteins), which were mainly involved in complement and coagulation cascades and glucose metabolism pathway, were identified by the proteomic experiments. Kininogen 1 (KNG1) was significantly increased (p < 0.0001), while apolipoprotein C-III (APOC3, p < 0.005) and paraoxonase 1 (PON1, p < 0.001) were significantly decreased in the serum of YDH syndrome rats. The combination of KNG1, APOC3, and PON1 constituted a diagnostic model with 100.0% sensitivity and 85.0% specificity. The results indicated that KNG1, APOC3, and PON1 may act as potential biomarkers for diagnosing YDH syndrome. KNG1 may regulate cytokines and chemokines release in YDH syndrome, and the low levels of PON1 and APOC3 may increase oxidative stress and lipolysis in YDH syndrome, respectively. Our work provides a novel method for YDH syndrome diagnosis and also provides valuable experimental basis to understand the molecular mechanism of YDH syndrome.
登录
查看更多内容
影响因子：
2.9
作者：
Diz, Angel P.;Truebano, Manuela;Skibinski, David O. F.
通讯作者：
Skibinski, David O. F.
影响因子：
4.8
作者：
Larsson, Mikael;Vorrsjo, Evelina;Olivecrona, Gunilla
通讯作者：
Olivecrona, Gunilla
影响因子：
3.5
作者：
Cao, Wen;Zhou, Yan;Liao, Ming
通讯作者：
Liao, Ming
影响因子：
8
作者：
Ning, C.;Li, Y-Y;Chen, G-J
通讯作者：
Chen, G-J
DOI：
10.1161/01.atv.0000240290.70852.c0
发表时间：
2006-10-01
影响因子：
8.7
作者：
Khan, Mohammad M.;Bradford, Harlan N.;Colman, Robert W.
通讯作者：
Colman, Robert W.