A transient expression system in soybean mesophyll protoplasts reveals the formation of cytoplasmic GmCRY1 photobody-like structures
A transient expression system in soybean mesophyll protoplasts reveals the formation of cytoplasmic GmCRY1 photobody-like structures
复制标题
大豆叶肉原生质体中的瞬时表达系统揭示了细胞质 GmCRY1 光体样结构的形成
DOI：
10.1007/s11427-018-9496-5
复制
发表时间：
2019-08-01
影响因子：
9.1
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Xiong, Lu;Li, Cong;Liu, Bin
大豆（甘氨酸max(l)）小麦，因其植物油和蛋白质而被种植，是世界上最重要的作物之一。培育稳定、可遗传的转基因大豆效率相对较低；因此，迫切需要一种简单高效的瞬时转化方法来研究大豆基因功能，从而阐明和完善相关农艺性状的分子调控机制。我们在大豆叶肉原生质体中建立了瞬时表达体系，获得了较高的原生质体转染效率（高达83.5%）。原生质体的亚细胞活性得到了很好的保存，这可以通过GmCRY核光体（NPs）和/或细胞质光体样结构（CPs）在蓝光下的动态形成来证明。此外，我们发现GmCRY1b CPs与拟南芥构成光形态基因1 （COP1）的同源基因GmCOP1b共定位，这为GmCRY1s在细胞质中的潜在作用提供了新的见解。
Soybean (Glycine max(L.) Merr.), grown for its plant oils and proteins, is one of the most important crops throughout the world. Generating stable and heritable transgenic soybeans is relatively inefficient; therefore, there is an urgent need for a simple and high-efficient transient transformation method by which to enable the investigation of gene functions in soybeans, which will facilitate the elucidation and improvement of the molecular mechanisms regulating the associated agronomic traits. We established a system of transient expression in soybean mesophyll protoplasts and obtained a high level of protoplast transfection efficiency (up to 83.5%). The subcellular activity of the protoplasts was well preserved, as demonstrated by the dynamic formation of GmCRY nucleus photobodies (NPs) and/or cytoplasmic photobody-like structures (CPs) in response to blue light. In addition, we showed that GmCRY1b CPs colocalized with GmCOP1b, a co-ortholog ofArabidopsis thalianaCONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1), which provided new insight into the potential roles of GmCRY1s in the cytoplasm.