Release of an inhibitor of angiogenesis upon induction of wild type p53 expression in glioblastoma cells
Release of an inhibitor of angiogenesis upon induction of wild type p53 expression in glioblastoma cells
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DOI：
10.1038/ng1094-171
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发表时间：
1994-10
期刊：
影响因子：
30.8
通讯作者：
Erwin G. Van Meir;P. Polverini;V. Chazin;H. Huang;N. Tribolet;W. Cavenee
中科院分区：
文献类型：
--
作者：
Erwin G. Van Meir;P. Polverini;V. Chazin;H. Huang;N. Tribolet;W. Cavenee
人类星形细胞瘤进展中最早的遗传改变是肿瘤抑制基因p53的突变，而最早的表型改变之一是刺激新生血管形成。在此，我们通过将四环素调控的野生型p53基因导入胶质母细胞瘤细胞中来检测p53在血管生成过程中的作用。亲本细胞表达强的血管生成活性，而在诱导野生型而非突变型p53表达时，细胞分泌能够中和由亲本细胞产生的因子以及碱性成纤维细胞生长因子的血管生成的因子。
The earliest genetic alteration in human astrocytoma progression is mutation of thep53tumour suppressor gene, while one of the earliest phenotypic changes is the stimulation of neovascularization. Here, we tested the role of p53 in the angiogenic process by introducing a tetracycline-regulated wild typep53gene into null glioblastoma cells. The parental cells expressed strong angiogenic activity while upon induction of wild type, but not mutant, p53 expression, the cells secreted a factor able to neutralize the angiogenicity of the factors produced by the parental cells as well as of basic fibroblast growth factor.