In-exercise vascular shear rate during acute continuous and interval exercise: impact on endothelial function and miR-21
In-exercise vascular shear rate during acute continuous and interval exercise: impact on endothelial function and miR-21
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DOI：
10.1152/japplphysiol.00156.2019
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发表时间：
2019-12-01
影响因子：
3.3
通讯作者：
Birch, Karen M.
中科院分区：
文献类型：
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作者：
Lyall, Gemma Kate;Davies, Matthew John;Birch, Karen M.
内皮细胞表型和内皮功能受血流动力学的调节，特别是壁切应力（WSS）。在单次运动中，特定的运动方案可以影响运动中的WSS模式，从而影响内皮功能。microRNA可能提供运动中WSS模式的生物标志物，以指示特定的运动回合是否会对内皮功能产生积极影响。我们评估了急性间歇（IT）和连续（CON）运动中WSS模式对运动后内皮功能和循环microRNA（miR）-21表达的影响。方法和结果：13名参与者进行CON和3种不同的IT锻炼协议，在不同的日子里进行持续时间和强度的匹配。在整个运动过程中记录氧摄取量、心率和肱动脉血流量。分别于运动前和运动后15 min进行肱动脉血流介导的舒张试验（FMD）。在运动前和运动后6 h采集血浆样品以测定miR-21表达。在运动剪切率（SR）模式（WSS的替代品）不同，根据CON或IT工作率配置文件。运动中CON组的顺向SR大于IT组（P <0.05），但两种运动方案的逆向SR相当（P> 0.05）。振荡剪切指数在间歇运动时高于对照运动时（P <0.05）。运动后FMD增加（治疗前：7.08%+/-2.95%，治疗后：10.54%+/-4.24%，P <0.05），而miR-21表达无变化（前：12.0%+/-20.7%cel-miR-39，后：11.1 +/-19.3%celmiR-39，P +/-0.05），运动方案无影响（P> 0.05）。结论：CON和IT运动诱导不同的SR模式，但同等的改善急性内皮功能。miR-21表达没有变化表明miR-21不是运动诱导SR的合适生物标志物。新&值得注意的是，由于血管剪切力的反复振荡，间隔运动有可能对血管适应产生负面影响。据我们所知，我们是第一个在不同的急性运动方案中连续评估运动诱导的剪切力，并检查其与急性内皮功能和剪切力循环生物标志物（miR-21）的关系。这些实验提供了明确的数据，表明不同的间隔运动方案的急性血管反应的增强，该研究还提供了详细的血管和剪切反应，供将来参考。
Endothelial cell phenotype and endothelial function are regulated by hemodynamic forces, particularly wall shear stress (WSS). During a single bout of exercise, the specific exercise protocol can affect in-exercise WSS patterns and, consequently, endothelial function. MicroRNAs might provide a biomarker of in-exercise WSS pattern to indicate whether a specific exercise bout will have a positive effect on endothelial function. We evaluated the effect of acute interval (IT) and continuous (CON) in-exercise WSS patterns upon postexercise endothelial function and circulating microRNA (miR)-21 expression. Methods and results: 13 participants performed CON and 3 different IT exercise protocols matched for duration and intensity on separate days. Oxygen uptake, heart rate, and brachial artery blood flow were recorded throughout the exercise. Brachial artery flow-mediated dilation (FMD) was performed pre-exercise and 15 min postexercise. Plasma samples were acquired pre-exercise and 6 h postexercise to determine miR-21 expression. In-exercise shear rate (SR) patterns (a surrogate of WSS) differed according to the CON or IT work-rate profile. In-exercise anterograde SR was greater in CON than IT exercise (P < 0.05), but retrograde SR was equivalent between exercise protocols (P > 0.05). Oscillatory shear index was higher during IT versus CON exercise (P < 0.05). Postexercise FMD increased (pre: 7.08% +/- 2.95%, post: 10.54% +/- 4.24%, P < 0.05), whereas miR-21 expression was unchanged (pre: 12.0% +/- 20.7% cel-miR-39, post: 11.1 +/- 19.3% celmiR-39, P +/- 0.05) with no effect of exercise protocol (P > 0.05). Conclusions: CON and IT exercise induced different SR patterns but equivalent improvements in acute endothelial function. The absence of change in miR-21 expression suggests that miR-21 is not a suitable biomarker of exercise-induced SR.NEW & NOTEWORTHY Interval exercise has the potential to negatively impact vascular adaptations because of repeated oscillations in vascular shear. To our knowledge, we are the first to continuously assess exercise-induced shear throughout different acute exercise protocols and examine its relationship with acute endothelial function and a circulating biomarker of shear (miR-21). These experiments provide clear data indicating enhancement of the acute vascular response from differing interval exercise protocols, with the study also providing detailed vascular and shear responses for future reference.