Breast milk contains relevant neurotrophic factors and cytokines for enteric nervous system development
Breast milk contains relevant neurotrophic factors and cytokines for enteric nervous system development
复制标题
DOI：
10.1002/mnfr.201100124
复制
发表时间：
2011-10-01
影响因子：
5.2
通讯作者：
Schaefer, Karl-Herbert
中科院分区：
文献类型：
--
作者：
Fichter, Michael;Klotz, Markus;Schaefer, Karl-Herbert
母乳喂养对于新生儿的发育起着重要作用。非母乳喂养的早产儿患婴儿腹泻和坏死性小肠结肠炎等疾病的风险明显更高。在这项研究中，测定了人母乳中可能影响肠神经系统（ENS）产后发育的神经营养因子和细胞因子的含量。使用单因子或多重 ELISA 分析胶质细胞系源性神经营养因子 (GDNF)、睫状神经营养因子 (CNTF) 以及一组细胞因子。为了将它们在牛奶中的存在与 ENS 发育的可能影响联系起来，在母乳的蛋白质提取物中培养大鼠肌间神经元。测量了神经胶质细胞中的神经突生长、神经元存活和巢蛋白表达。所有母乳样本中均发现了不同浓度的生长因子和细胞因子。可以证明，母乳的蛋白质提取物增加了存活的肠神经元以及神经突生长的数量。此外，研究还表明，在母乳蛋白提取物中孵育后，表达巢蛋白和 S100 的神经胶质细胞的数量显着增加。数据表明，牛奶中的蛋白质支持肠道神经系统的发育。
Breast-feeding plays an important role for the development of the newborn. Non-breast fed premature born infants show a significantly higher risk of developing diseases like infantile diarrhoea and necrotizing enterocolitis. In this study, the content of neurotrophic factors and cytokines, which might influence the postnatal development of the enteric nervous system (ENS), was determined in human breast milk. Glial cell-line-derived neurotrophic factor (GDNF), ciliary neurotrophic factor (CNTF) as well as a panel of cytokines were analyzed using single factor or multiplex ELISA. In order to link their presence in milk with possible effects on the development of the ENS, rat myenteric neurons were cultured in protein extracts from breast milk. Neurite outgrowth, neuron survival and nestin expression in glial cells were measured. Growth factors and cytokines were found in all breast milk samples at varying concentrations. It could be demonstrated that protein extracts of breast milk increased the amount of surviving enteric neurones as well as neurite outgrowth. Additionally it was shown, that the number of nestin and S100-expressing glial cells increased significantly after incubating in breast milk protein extracts. The data suggest that milk-born proteins support the development of the enteric nervous system.