Co-localization of Pirt protein and P2X2 receptors in the mouse enteric nervous system
Co-localization of Pirt protein and P2X2 receptors in the mouse enteric nervous system
复制标题
Pirt 蛋白和 P2X2 受体在小鼠肠神经系统中的共定位。
DOI：
10.1007/s11302-016-9515-6
复制
发表时间：
2016-09-01
影响因子：
3.5
通讯作者：
Xiang, Zhenghua
中科院分区：
文献类型：
--
作者：
Guo, Wei;Sui, Qian-Qian;Xiang, Zhenghua
P2X2受体与其他P2X受体亚型在调节胃肠道功能中介导突触传递具有重要作用。我们最近的工作发现了一种新的P2X受体功能调节剂，称为瞬时受体电位通道磷酸肌醇相互作用调节剂（pit）。在目前的工作中，我们已经表明，Pirt免疫反应性局限于胃、回肠、结肠近端和远端肌肠丛的神经细胞体和神经纤维，以及空肠、回肠、结肠近端和远端粘膜下丛。几乎所有的Pirt-immunoreactive （ir）神经元也是P2X2-ir，共免疫沉淀实验表明，Pirt与抗p2x2抗体共沉淀。这项工作提供了关于肠道中port表达及其与P2X2共定位的详细信息，表明其在影响P2X2受体功能方面的潜在作用。
P2X2 receptors, with other P2X receptor subtypes, have an important role mediating synaptic transmission in regulating the functions of the gastrointestinal tract. Our recent work has found a new regulator of P2X receptor function, called phosphoinositide-interacting regulator of transient receptor potential channels (Pirt). In the present work, we have shown that Pirt immunoreactivity was localized in nerve cell bodies and nerve fibers in the myenteric plexus of the stomach, ileum, proximal, and distal colon and in the submucosal plexus of the jejunum, ileum, proximal, and distal colon. Almost all the Pirt-immunoreactive (ir) neurons were also P2X2-ir, and co-immunoprecipitation experiments have shown that Pirt co-precipitated with the anti-P2X2 antibody. This work provides detailed information about the expression of Pirt in the gut and its co-localization with P2X2, indicating its potential role in influencing P2X2 receptor function.