In vivo and in vitro biofilm formation on two different titanium implant surfaces
In vivo and in vitro biofilm formation on two different titanium implant surfaces
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DOI：
10.1111/j.1600-0501.2009.01815.x
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发表时间：
2010-02-01
影响因子：
4.3
通讯作者：
Gosau, Martin
中科院分区：
文献类型：
--
作者：
Buergers, Ralf;Gerlach, Till;Gosau, Martin
目的本体外和人体体内研究的目的有两个：首先，通过两种高灵敏度的荧光技术评估不同钛植入物表面上的初始生物膜形成，其次，将这些发现与不同的表面性质相关联。材料和方法在纯机械加工（Pt）和喷砂和酸蚀刻的钛（Prom）样品上诱导体内生物膜形成，将其颊部安装在单独的夹板上，并由六名研究参与者佩戴12小时。还在与血链球菌悬浮液孵育（37 ℃，2小时）后研究了体外细菌粘附。通过以下荧光技术定量粘附细菌：刃天青染色结合自动荧光读数器或活/死细胞标记和荧光显微镜。表面粗糙度（R-a）与perthometer确定，和表面自由能（SFE）与测角器。ResultsProm显示出显着较高的中位数R-a（0.95 μ m）和显着较低的中位数SFE（18.3 mJ/m2）比铂（R-a =0.15 μ m; SFE=39.6 mJ/m2）。在体外和体内的测试表明，一个显着更高的细菌粘附Prom比Pt，和Pt上的初始生物膜形成对应的圆形表面改性的加工基层。这两种观察结果可能归因于表面粗糙度对细菌粘附的主要影响。所有粘附细菌中的死细胞百分比在Prom（23.7%）和Pt（29.1%）之间没有显著差异。异位孤立的上皮细胞从口腔粘膜-强烈粘附的底层-被发现在每个Prom标本，但不是在任何的Pt surfaces.ConclusionsInitial细菌粘附到不同纹理的钛表面主要是由R-a的影响，而SFE的影响似乎只有轻微的重要性。因此，暴露于口腔的植入物的微结构部分应高度抛光以防止牙菌斑积聚。两种测试的荧光技术被证明是高度敏感和可重复的定量生物膜形成的钛种植体surface.To引用这篇文章：Burgers R，盖拉赫T，Hahnel S，施瓦茨F，Handel G，Gosau M.两种不同钛种植体表面生物膜形成的体内和体外研究。Res. 21，2010; 156-164doi：10.1111/j.1600-0501.2009.01815.x.
ObjectivesThe aim of the present in vitro and human in vivo study was twofold: first, to evaluate the initial biofilm formation on different titanium implant surfaces by means of two highly sensitive fluorescent techniques and, second, to correlate these findings to different surface properties.Materials and methodsIn vivo biofilm formation was induced on purely machined (Pt) and on sand-blasted and acid-etched titanium (Prom) specimens, which were mounted buccally on individual splints and worn by six study participants for 12 h. In vitro bacterial adhesion was also investigated after incubation with Streptococcus sanguinis suspension (37 degrees C, 2 h). Adherent bacteria were quantified by the following fluorescence techniques: Resazurin staining in combination with an automated fluorescence reader or live/dead cell labeling and fluorescence microscopy. Surface roughness (R-a) was determined with a perthometer, and surface free energy (SFE) was measured with a goniometer.ResultsProm showed a significantly higher median R-a (0.95 mu m) and a significantly lower median SFE (18.3 mJ/m2) than Pt (R-a=0.15 mu m; SFE=39.6 mJ/m2). The in vitro and in vivo tests showed a significantly higher bacterial adhesion to Prom than to Pt, and the initial biofilm formation on Pt corresponded to the circular surface modifications on the machined substratum. Both observations may be attributed to the predominant influence of surface roughness on bacterial adhesion. No significant differences in the percentage of dead cells among all adhering bacteria were found between Prom (23.7%) and Pt (29.1%). Ectopic solitary epithelial cells from the oral mucosa - strongly adhering to the substratum - were found on each Prom specimen, but not on any of the Pt surfaces.ConclusionsInitial bacterial adhesion to differently textured titanium surfaces is primarily influenced by R-a, whereas the influence of SFE seems to be of only minor importance. Therefore, the micro-structured parts of an implant that are exposed to the oral cavity should be highly polished to prevent plaque accumulation. Both tested fluorometric techniques proved to be highly sensitive and reproducible in the quantification of biofilm formation on titanium implant surfaces.To cite this article:Burgers R, Gerlach T, Hahnel S, Schwarz F, Handel G, Gosau M. In vivo and in vitro biofilm formation on two different titanium implant surfaces.Clin. Oral Impl. Res. 21, 2010; 156-164doi: 10.1111/j.1600-0501.2009.01815.x.