Evidence that auxin is required for normal seed size and starch synthesis in pea
Evidence that auxin is required for normal seed size and starch synthesis in pea
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DOI：
10.1111/nph.14690
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发表时间：
2017-10-01
期刊：
影响因子：
9.4
通讯作者：
Ross, John J.
中科院分区：
文献类型：
--
作者：
McAdam, Erin L.;Meitzel, Tobias;Ross, John J.
近年来，借助生长素生物合成基因的突变，生长素的生物合成得到了澄清。然而，我们对这些突变对种子发育的灌浆期的影响知之甚少。在这里，我们研究了一个关键的植物生长素生物合成突变，导致生长中的豌豆种子缺乏生长素。我们利用这种模式物种的大种子大小，这有利于测量单个种子中的化合物。该突变导致种子小，淀粉含量降低，干燥期呈褶皱表型。该突变的表型效应被野生型基因作为转基因引入后完全逆转，被生长素应用后部分逆转。结果表明，作为一种重要的豆科作物，豌豆需要生长素来维持正常的种子大小和淀粉积累。
In recent years the biosynthesis of auxin has been clarified with the aid of mutations in auxin biosynthesis genes. However, we know little about the effects of these mutations on the seed-filling stage of seed development.Here we investigate a key auxin biosynthesis mutation of the garden pea, which results in auxin deficiency in developing seeds. We exploit the large seed size of this model species, which facilitates the measurement of compounds in individual seeds.The mutation results in small seeds with reduced starch content and a wrinkled phenotype at the dry stage. The phenotypic effects of the mutation were fully reversed by introduction of the wild-type gene as a transgene, and partially reversed by auxin application.The results indicate that auxin is required for normal seed size and starch accumulation in pea, an important grain legume crop.