Vaccinia virus K1L protein mediates host-range function in RK-13 cells via ankyrin repeat and may interact with a cellular GTPase-activating protein
Vaccinia virus K1L protein mediates host-range function in RK-13 cells via ankyrin repeat and may interact with a cellular GTPase-activating protein
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DOI：
10.1016/j.virusres.2005.06.003
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发表时间：
2005-12-01
期刊：
影响因子：
5
通讯作者：
Terajima, M
中科院分区：
文献类型：
--
作者：
Bradley, RR;Terajima, M
牛痘病毒的KL蛋白是其在某些细胞系（RK- 13和人）中生长所必需的。牛痘宿主范围蛋白CP 77已经显示出在RK- 13细胞中补充KIL功能，尽管除了锚蛋白重复序列之外，这两种蛋白质之间缺乏同源性。我们研究了KIL蛋白的锚蛋白重复序列在RK- 13细胞中的作用。KIL基因在最保守的锚蛋白重复序列中发生突变的重组痘苗病毒的生长严重受损。感染突变病毒导致细胞和病毒蛋白质合成在感染早期关闭。我们还研究了KIL蛋白与细胞蛋白的相互作用，发现KIL与人ACAP 2的兔同源物相互作用，人ACAP 2是一种具有锚蛋白重复序列的GTP酶激活蛋白。我们的研究结果表明锚蛋白重复序列对RK-13细胞中KIL的宿主范围功能的重要性，并将ACAP 2鉴定为可能与KIL相互作用的细胞蛋白。(c)2005 Elsevier B. V.保留所有权利。
The K I L protein of vaccinia virus is required for its growth in certain cell lines (RK- 13 and human). The cowpox host-range protein CP77 has been shown to complement K I L function in RK- 13 Cells, despite a lack of homology between the two proteins except for ankyrin repeats. We investigated the role of ankyrin repeats of K I L protein in RK- 13 cells. The growth of a recombinant vaccinia virus, with KIL gene mutated in the most conserved ankyrin repeat, was severely impaired. Infection with the mutant virus caused shutdown of cellular and viral protein synthesis early in infection. We also investigated the interaction of K I L protein with cellular proteins and found that K I L interacts with the rabbit homologue of human ACAP2, a GTPase-activating protein with ankyrin repeats. Our result suggests the importance of ankyrin repeat for host-range function of KIL in RK-13 cells and identifies ACAP2 as a cellular protein, which may be interacting with KIL. (c) 2005 Elsevier B.V. All rights reserved.