Novel chitosan wound dressing loaded with minocycline for the treatment of severe burn wounds
Novel chitosan wound dressing loaded with minocycline for the treatment of severe burn wounds
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DOI：
10.1016/j.ijpharm.2006.09.016
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发表时间：
2007-02-07
影响因子：
5.8
通讯作者：
Machida, Yoshiharu
中科院分区：
文献类型：
--
作者：
Aoyagi, Shuichi;Onishi, Hiraku;Machida, Yoshiharu
以市售聚氨酯膜（Tegadaline）为背衬，制备了由壳聚糖（CH）膜和盐酸米诺环素（MH）组成的新型伤口敷料。使用脱乙酰度为67%、83%和96%（mol/mol）的CH，分别命名为CH 67、CH 83和CH 96。开发了具有大片Tegadidine膜（4 cm x 4 cm）的伤口敷料（命名为CH-MH-N）和通过将CH-MH-N切割至伤口尺寸制备的伤口敷料（命名为CH-MH-A）。由于CH 67-MH-N和CH 83-MH-N在体外显示米诺环素的缓释，因此在体内研究中使用CH 67和CH 83作为壳聚糖。将各种制剂在早期应用于大鼠严重烧伤创面，并检查创面状态和创面表面积的变化。使用10 mg MH和Tegad完全封闭有负面影响。MH软膏无效，格本乳膏疗效较好。然而，含有2 mg MH的CH 83-MH-A（CH 83-MH 2-A）和CH 83膜显示出优异的效果。从排脓效果来看，CH 83-MH 2-A膜优于CH 83膜。CH 83-MH 2-A被认为是用于治疗严重烧伤伤口的有用制剂。(c)2006 Elsevier B. V.保留所有权利。
Novel wound dressings composed of chitosan (CH) film and minocycline hydrochloride (MH) were prepared using commercial polyurethane film (Tegaderm) as a backing. CHs with deacetylation degrees of 67%, 83% and 96% (mol/mol), named CH67, CH83 and CH96, respectively, were used. Wound dressing with a large piece of Tegaderm film (4 cm x 4 cm), named CH-MH-N, and wound dressing prepared by cutting CH-MH-N to the wound size, named CH-MH-A, were developed. As CH67-MH-N and CH83-MH-N showed the sustained release of minocycline in vitro, CH67 and CH83 were used as chitosan in the in vivo studies. Various formulations were applied to severe burn wounds in rats in the early stage, and the wound status and change in the wound surface area were examined. The use of 10 mg of MH and complete sealing with Tegaderm had a negative effect. MH ointment was not effective, but Geben cream was fairly effective. However, CH83-MH-A containing 2 mg of MH (CH83-MH2-A) and CH83 film showed an excellent effect. Considering the elimination of pus, CH83-MH2-A tended to be better than CH83 film. CH83-MH2-A is suggested as a useful formulation for the treatment of severe burn wounds. (c) 2006 Elsevier B.V. All rights reserved.