GLYCOLYSIS IS PREDOMINANT SOURCE OF MYOCARDIAL ATP PRODUCTION IMMEDIATELY AFTER BIRTH
GLYCOLYSIS IS PREDOMINANT SOURCE OF MYOCARDIAL ATP PRODUCTION IMMEDIATELY AFTER BIRTH
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DOI：
10.1152/ajpheart.1991.261.6.h1698
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发表时间：
1991-12-01
影响因子：
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通讯作者：
MARSH, DR
中科院分区：
文献类型：
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作者：
LOPASCHUK, GD;SPAFFORD, MA;MARSH, DR
测定了来自1日龄和7日龄兔的离体工作心脏的糖酵解通量，以及葡萄糖、脂肪酸和乳酸氧化。1日龄兔心脏通过下腔静脉灌流，主动脉和肺动脉后负荷恒定，而7日龄兔心脏通过左心房灌流，主动脉后负荷恒定。用含有100mU/ml胰岛素和1)11 mM[U-C-14/2-H-3]葡萄糖、0.4 mM棕榈酸酯、2 mM乳酸盐的缓冲液；2)11 mM葡萄糖、0.4 mM[1-C-14]棕榈酸酯、2 mM乳酸盐；或3)11 mM葡萄糖、0.4 mM棕榈酸酯、2 mM[U-C-14]乳酸盐。糖酵解率(测量为(H2O)-H-3产生)在1日龄的心脏中很高，但在7日龄时下降(从2730+/-280降至580+/-80nmol.min-1.g干重量-1)。1日龄和7日龄心脏的葡萄糖氧化率(测量为(CO2)-C-14产生)都较低(59+/-4.4和23+/-2nmol.min-1.g干重)。棕榈酸氧化速率在1天的心脏中很低，但在7天后急剧增加(分别为22.6+/-5.6和305+/-33nmol.min-1.g干重)。相比之下，乳酸盐很容易被1天和7天的心脏氧化(分别为169+/-14和456+/-52nmol.min-1.g干重量-1)。在1日龄的心脏中，44%的稳定的外源ATP产生来自糖酵解，而18%、13%和25%分别来自葡萄糖、棕榈酸和乳酸的氧化。在7日龄的心脏中，外源产生的ATP只有7%来自糖酵解，分别有5%、39%和49%来自葡萄糖、棕榈酸酯和乳酸氧化。这些结果表明，在兔出生后立即，糖酵解是心脏产生外源ATP的主要来源，而脂肪酸不容易被氧化。然而，在出生后的几天内，作为主要能量生产来源的糖酵解发生了戏剧性的变化，脂肪酸和乳酸氧化都是主要的能量产生来源。
Glycolytic flux, as well as glucose, fatty acid, and lactate oxidation, was determined in isolated working hearts obtained from 1- and 7-day-old rabbits. One-day-old rabbit hearts were perfused via the inferior cava against a constant aortic and pulmonary arterial afterload, whereas hearts from 7-day-old rabbits were perfused via the left atria against a constant aortic afterload. Hearts were perfused with buffer containing 100-mu-U/ml insulin and either 1) 11mM [U-C-14/2-H-3]glucose, 0.4 mM palmitate, 2 mM lactate; 2) 11 mM glucose, 0.4 mM [1-C-14]palmitate, 2 mM lactate; or 3) 11 mM glucose, 0.4 mM palmitate, 2 mM [U-C-14]lactate. Glycolytic rates (measured as (H2O)-H-3 production) were high in 1-day-old hearts but decreased by 7 days (from 2,730 +/- 280 to 580 +/- 80 nmol.min-1.g dry wt-1). Rates of glucose oxidation (measured as (CO2)-C-14 production) were lower in both 1- and 7-day-old hearts (59 +/- 4.4 and 23 +/- 2 nmol.min-1.g dry wt-1). Palmitate oxidation rates were low in 1-day-old hearts but dramatically increased by 7 days (22.6 +/- 5.6 and 305 +/- 33 nmol oxidized.min-1.g dry wt-1, respectively). In contrast, lactate was readily oxidized by both 1- and 7-day-old hearts (169 +/- 14 and 456 +/- 52 nmol.min-1.g dry wt-1, respectively). In 1-day-old hearts, 44% of steady-state ATP production from exogeneous sources were derived from glycolysis, whereas 18, 13, and 25% were derived from glucose, palmitate, and lactate oxidation, respectively. In 7-day-old hearts, only 7% of ATP production from exogenous sources were derived from glycolysis, with 5, 39, and 49% being derived from glucose, palmitate, and lactate oxidation, respectively. These results demonstrate that immediately after birth in the rabbit, glycolysis is the major source of exogenous ATP production in the heart, whereas fatty acids are not readily oxidized. Within days of birth, however, there is a dramatic shift from glycolysis to both fatty acid and lactate oxidation as the primary sources of energy production.