Ion channels in plasmalemma of wheat protoplasts.
Ion channels in plasmalemma of wheat protoplasts.
复制标题
小麦原生质体质膜中的离子通道。
作者：
N. Moran;G. Ehrenstein;K. Iwasa;C. Bare;C. Mischke
应用膜片钳技术研究了离子在小麦叶片原生质体质膜中的被动运动。这种方法克服了传统植物细胞电生理研究中固有的问题。记录从质膜上切下的斑片的电导变化。观察到两种类型的斑块：（i）低通道密度区域，其中离散的单通道电流可以被分辨，电导范围为10至200皮西门子;（ii）高通道密度区域，其中单通道电流不能被分辨，电导为几纳西门子。结果表明，动物和植物细胞膜之间的运输的基本现象惊人的相似性。此外，所使用的方法构成了一个新的精细化程度的研究过程中的调节，病理学和毒性的植物。
The patch-clamp technique was used to study passive movements of ions through the plasmalemma of wheat leaf protoplasts. This method overcomes the problems inherent in conventional electrophysiological study of plant cells. Changes in conductance were recorded in patches excised from the plasmalemma. Two types of patches were observed: (i) regions of low channel density, where discrete single-channel currents could be resolved and conductance ranged from 10 to 200 picosiemens and (ii) regions of high channel density, where single-channel currents could not be resolved and conductance was on the order of a few nanosiemens. The results indicate a striking similarity between animal and plant cell membranes in the basic phenomena of transport. Moreover, the approach used constitutes a new degree of refinement in the study of processes of regulation, pathology, and toxicity in plants.