Quantitative GSTP1 methylation clearly distinguishes benign prostatic tissue and limited prostate adenocarcinoma
Quantitative GSTP1 methylation clearly distinguishes benign prostatic tissue and limited prostate adenocarcinoma
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DOI：
10.1097/01.ju.0000049627.90307.4d
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发表时间：
2003-03-01
影响因子：
6.6
通讯作者：
Sidransky, D
中科院分区：
文献类型：
--
作者：
Harden, SV;Guo, Z;Sidransky, D
目的：谷胱甘肽S转移酶基因高甲基化是前列腺癌中最常见的表观遗传学改变(90%以上)。它发生在癌症进展的早期，是检测器官受限疾病的一个有前途的标记物。为了评价其作为癌症诊断工具的价值，我们采用定量GSTP1甲基化检测了45例前列腺针刺活检标本中癌的存在。材料和方法：采用荧光实时甲基化特异性聚合酶链式反应定量检测45例前列腺癌或良性疾病患者的石蜡组织标本中GSTP1的甲基化状态。结果：45份石蜡标本中有29份DNA能进行聚合酶链式反应扩增。在这29例标本中，15例局限性癌中11例检出GSTP1甲基化，14例良性病变中0例检出GSTP1甲基化(双侧Fisher‘s精确检验，P
Purpose: Hypermethylation of the glutathione S-transferase gene (GSTP1) is the most common (greater than 90%) reported epigenetic alteration in prostate cancer. It occurs early in cancer progression and it is a promising marker for detecting organ confined disease. To evaluate its use as a diagnostic tool for cancer we used quantitative GSTP1 methylation to test for the presence of cancer in 45 prostate needle biopsy samples.Materials and Methods: Paraffin tissue samples from 45 patients with minute foci of intermediate grade prostatic adenocarcinoma or benign disease on needle biopsy were tested for GSTP1 hypermethylation using quantitative fluorogenic real-time methylation specific polymerase chain reaction. This assay was performed in blinded fashion without previous knowledge of the histopathological diagnosis.Results: DNA from 29 of the 45 paraffin samples was amenable to polymerase chain reaction amplification. In these 29 samples GSTP1 methylation was detected in 11 of 15 cases of limited cancer and in 0 of 14 of benign disease (2-sided Fisher's exact test, p