Quantitative evaluation of magnetic immunoassay with remanence measurement
Quantitative evaluation of magnetic immunoassay with remanence measurement
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DOI：
10.1088/0953-2048/19/5/s19
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发表时间：
2006-05-01
影响因子：
3.6
通讯作者：
Kandori, A
中科院分区：
文献类型：
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作者：
Enpuku, K;Soejima, K;Kandori, A
利用磁性标记物和高T-C SQUID的磁性免疫测定已经进行。设计标记以产生剩磁，并且用SQUID测量其剩磁场。SQUID系统的开发是为了在一个测量序列中测量12个样品。我们首先使用IgE标准溶液进行称为人IgE的抗原的检测，并且显示IgE的检测低至2阿托摩尔。IgE与标记物的结合过程可用Langmuir型吸附模型半定量解释。我们还测量了人血清中的IgE，并证明了本方法在实际诊断中的实用性。
Magnetic immunoassays utilizing magnetic markers and a high-T-c SQUID have been performed. The marker was designed so as to generate remanence, and its remanence field was measured with the SQUID. The SQUID system was developed so as to measure 12 samples in one measurement sequence. We first conducted a detection of antigen called human IgE using IgE standard solution, and showed the detection of IgE down to 2 attomol. The binding process between IgE and the marker could be semi-quantitatively explained with the Langmuir-type adsorption model. We also measured IgE in human serums, and demonstrated the usefulness of the present method for practical diagnosis.