Combgap contributes to recruitment of Polycomb group proteins in Drosophila.
Combgap contributes to recruitment of Polycomb group proteins in Drosophila.
复制标题
Combgap 有助于果蝇中多梳族蛋白的募集。
DOI：
10.1073/pnas.1520926113
复制
发表时间：
2016
影响因子：
11.1
通讯作者：
Kassis,JudithA
中科院分区：
文献类型：
--
作者：
Ray,Payal;De,Sandip;Mitra,Apratim;Bezstarosti,Karel;Demmers,JeroenAA;Pfeifer,Karl;Kassis,JudithA
Polycomb group (PcG) proteins are responsible for maintaining the silenced transcriptional state of many developmentally regulated genes. PcG proteins are organized into multiprotein complexes that are recruited to DNA viacis-acting elements known as “Polycomb response elements” (PREs). InDrosophila, PREs consist of binding sites for many different DNA-binding proteins, some known and others unknown. Identification of these DNA-binding proteins is crucial to understanding the mechanism of PcG recruitment to PREs. We report here the identification of Combgap (Cg), a sequence-specific DNA-binding protein that is involved in recruitment of PcG proteins. Cg can bind directly to PREs via GTGT motifs and colocalizes with the PcG proteins Pleiohomeotic (Pho) and Polyhomeotic (Ph) at the majority of PREs in the genome. In addition, Cg colocalizes with Ph at a number of targets independent of Pho. Loss of Cg leads to decreased recruitment of Ph at only a subset of sites; some of these sites are binding sites for other Polycomb repressive complex 1 (PRC1) components, others are not. Our data suggest that Cg can recruit Ph in the absence of PRC1 and illustrate the diversity and redundancy of PcG protein recruitment mechanisms.
