IMMUNOHISTOCHEMICAL CHARACTERIZATION OF SIALADENITIS IN NZBXNZW-F1 MICE
IMMUNOHISTOCHEMICAL CHARACTERIZATION OF SIALADENITIS IN NZBXNZW-F1 MICE
复制标题
DOI：
10.1016/0090-1229(87)90176-0
复制
发表时间：
1987-01-01
期刊：
影响因子：
--
通讯作者：
KLARESKOG, L
中科院分区：
文献类型：
--
作者：
JONSSON, R;TARKOWSKI, A;KLARESKOG, L
在女性自身免疫性NZB中自发发展的涎腺炎。NZW F1（NZB/W）小鼠已研究的帮助下，免疫组织化学和单克隆抗体的细胞表面抗原。半定量评估染色细胞内浸润揭示了一个渐进性局灶性炎症最明显的下颌下腺和腮腺。大多数细胞表达Ly-1（所有T细胞）和L3 T4（辅助性T细胞）表型，而仅观察到少数Lyt-2（细胞毒性/抑制性）表达T细胞。大部分浸润细胞的Ia抗原染色，这也被发现在唾液腺导管上皮淋巴浸润附近。因此，NZB/W小鼠的涎腺炎的表型模式与先前描述的人类舍格伦综合征（SS）的模式非常相似。因此，NZB/W涎腺炎的免疫形态学分析可能有助于进一步研究实验和人类SS的发病机制和治疗。
The spontaneously developing sialadenitis in female autoimmune NZB .times. NZW F1 (NZB/W) mice has been studied with the help of immunohistochemistry and monoclonal antibodies to cell surface antigens. Semiquantitative assessment of stained cells within the infiltrates disclosed a progressive focal inflammation most pronounced in submandibular and parotid glands. The majority of cells expressed Ly-1 (all T cells) and L3T4 (T helper) phenotype, whereas only few Lyt-2 (cytotoxic/suppressor) expressing T cells were seen. A large proportion of the infiltrating cells stained for Ia antigens, which was also found on salivary gland ductal epithelium in the proximity of lymphoid infiltrates. The phenotypic pattern in sialadenitis of NZB/W mice thus closely resembles the pattern previously described for human Sjorgren''s syndrome (SS). Acordingly, immunomorphological analysis of the NZB/W sialadenitis may be useful in further studies of pathogenesis and therapy of both experimental and human SS.