Upregulation of interferon-gamma-induced genes during prion infection.
Upregulation of interferon-gamma-induced genes during prion infection.
复制标题
DOI：
10.1080/15287394.2011.529064
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Aiken JM
中科院分区：
文献类型：
--
作者：
Moody LR;Herbst AJ;Aiken JM
全局基因表达分析允许鉴定在疾病状态期间差异调节的转录物。许多研究小组，包括我们自己的研究小组，已经确定了数百个在朊病毒感染反应中差异调节的基因。在朊病毒感染的动物的脑中上调的11种转录物被鉴定，其在文献中被分类为受细胞因子干扰素-γ（IFN-γ）刺激。这很有趣，因为最近在朊病毒感染动物的大脑中检测到了IFN-γ。通过定量实时聚合酶链反应（qRT-PCR）对归类为IFN-γ诱导型的几种基因进行定量，证实这些转录物上调。未来的方法来描绘IFN-γ诱导的转录物的作用和它们在朊病毒感染中的功能。
Global gene expression analysis allows for the identification of transcripts that are differentially regulated during a disease state. Many groups, including our own, have identified hundreds of genes differentially regulated in response to prion infection. Eleven transcripts, upregulated in the brains of prion-infected animals, which were classified in the literature as stimulated by the cytokine interferon-gamma (IFN-γ ), were identified. This is intriguing, as IFN-γ has recently been detected in the brains of prion-infected animals. Quantitation of several genes, categorized as IFN-γ inducible, by quantitative real-time polymerase chain reaction (qRT-PCR) confirms that these transcripts are upregulated. Future approaches for delineating the role of IFN-γ -induced transcripts and their function in prion infection are described.