CENTRIOLE CYCLE IN CHINESE-HAMSTER OVARY CELLS AS DETERMINED BY WHOLE-MOUNT ELECTRON-MICROSCOPY
CENTRIOLE CYCLE IN CHINESE-HAMSTER OVARY CELLS AS DETERMINED BY WHOLE-MOUNT ELECTRON-MICROSCOPY
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DOI：
10.1083/jcb.91.3.814
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发表时间：
1981-01-01
影响因子：
7.8
通讯作者：
BORISY, GG
中科院分区：
文献类型：
--
作者：
KURIYAMA, R;BORISY, GG
在间期中国仓鼠卵巢 (CHO) 细胞中，中心体非常牢固地附着在细胞核上。通过在低离子强度介质中用 Triton X-100 裂解和提取细胞，将这种核中心体复合物分离为连贯结构。在这些条件下，可以通过整体制备物的电镜来辨别附着在细胞核上的中心粒的超微结构。通过该技术监测中心粒作为细胞周期函数的结构变化。根据中心粒的方向以及子代和亲代中心粒的长度比，中心粒轮廓分为 6 类。每个类别中中心粒的比例被绘制为频率直方图。中心粒循环中的形态变化以 3 个可区分的事件为特征：成核、伸长和定向障碍。中心粒在这些阶段的进展是在从 S 或 M 期开始的同步细胞群中、在通过添加胸苷抑制 DNA 合成的细胞中以及在细胞质中确定的。提供了中心粒循环事件的定量描述。在完整的细胞中，成核、伸长和定向障碍不依赖于 DNA 合成。在细胞质中，尽管与正常细胞一样发生伸长和定向障碍，但成核被阻止。原中心粒的形成似乎因细胞核的去除而受到抑制。显然，中心粒复制和核复制的协调可能取决于来自细胞核的信号。
In interphase Chinese hamster ovary (CHO) cells, the centrosome is attached to the nucleus very firmly. This nuclear-centrosome complex is isolated as a coherent structure by lysis and extraction of cells with Triton X-100 in a low ionic strength medium. Under these conditions the ultrastructure of the centrioles attached to the nucleus can be discerned by EM of whole-mount preparations. The structural changes of the centrioles as a function of the cell cycle were monitored by this technique. Centriolar profiles were placed into 6 categories according to their orientation and the length ratio of daughter and parent centrioles. The proportion of centrioles in each category was plotted as a frequency histogram. The morphological changes in the centriole cycle were characterized by 3 distinguishable events: nucleation, elongation and disorientation. The progress of centrioles through these stages was determined in synchronous populations of cells starting from S or M phase, in cells inhibited in DNA synthesis by addition of thymidine, and in cytoplasts. A quantitative description of the events of the centriole cycle is provided. In complete cells, nucleation, elongation and disorientation are not dependent upon DNA synthesis. In cytoplasts, although elongation and disorientation occur as in normal cells, nucleation is blocked. Procentriole formation appeared to be inhibited by removal of the nucleus. Apparently coordination of centriole replication and nuclear replication may depend upon a signal arising from the nucleus.