Hypoxia up-regulates vascular endothelial growth factor in U-87 MG cells: Involvement of TRPC1
Hypoxia up-regulates vascular endothelial growth factor in U-87 MG cells: Involvement of TRPC1
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缺氧上调 U-87 MG 细胞中的血管内皮生长因子：TRPC1 的参与
DOI：
10.1016/j.neulet.2009.05.015
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发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
王斌
中科院分区：
文献类型：
--
作者：
邹飞;孟晓静;李文军;王斌
经典瞬时受体电位（TRPC）通道在多种生理过程中发挥重要作用。在恶性胶质瘤细胞系U-87 MG细胞中研究了TRPC通道在缺氧条件下上调VEGF表达的贡献。TRPC通道阻断剂可显著抑制缺氧引起的VEGF基因表达上调。RT-PCR结果显示，U-87 MG细胞在常氧条件下表达TRPC的4种亚型：TRPC 1、3、4和5。此外，TRPC 3，4和5的表达在缺氧暴露下大大降低U-87 MG细胞。相反，TRPC 1表达没有变化。这些结果表明TRPC通道参与了缺氧诱导的VEGF表达，与其他TRPC亚型相比，TRPC 1可能在此过程中发挥不同的作用。此外，我们还利用RNAi技术对TRPC 1的功能进行了研究。针对TRPC 1的两种不同siRNA在很大程度上抑制了缺氧诱导的VEGF mRNA和蛋白水平的上调。然而，过度表达TRPC 3或5既不增强缺氧诱导的VEGF表达，也不阻止它。两者合计，我们目前的数据表明，TRPC 1，而不是TRPC 3或5，参与缺氧诱导的VEGF表达在U-87 MG细胞。
Canonical Transient Receptor Potential (TRPC) channels play important roles in diverse physiological processes. The contribution of TRPC channels to up-regulate VEGF expression under hypoxic conditions was studied in a malignant glioma cell line, U-87 MG cells. Up-regulation of VEGF gene expression by hypoxia was markedly suppressed by a TRPC channel blocker. RT-PCR showed that U-87 MG cells expressed four TRPC isoforms in normoxia: TRPC1, 3, 4, and 5. In addition, the expression of TRPC3, 4, and 5 decreased greatly under hypoxia exposure in U-87 MG cells. In contrast, TRPC1 expression was unchanged. These results suggest TRPC channels were involved in hypoxia-induced VEGF expression, and compared with other TRPC isoforms, TRPC1 might play a different role in this process. Furthermore, we determined the function of TRPC1 by RNAi. Two different siRNAs against TRPC1 largely inhibited hypoxia-induced up-regulation of VEGF mRNA and protein levels. However, overexpression of TRPC3 or 5 neither enhanced hypoxia-induced VEGF expression, nor prevented it. Taken together, our present data suggest that TRPC1, but not TRPC3 or 5, is involved in hypoxia-induced VEGF expression in U-87 MG cells.
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影响因子：
9.3
作者：
Eleni Zygalaki;A. Stathopoulou;C. Kroupis;L. Kaklamanis;Z. Kyriakides;D. Kremastinos;E. Lianidou
通讯作者：
Eleni Zygalaki;A. Stathopoulou;C. Kroupis;L. Kaklamanis;Z. Kyriakides;D. Kremastinos;E. Lianidou
影响因子：
2.3
作者：
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通讯作者：
F. Meng;W. To;Yuchun Gu
DOI：
10.1073/pnas.191352298
发表时间：
2001-09-25
影响因子：
11.1
作者：
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通讯作者：
Greenberg, ME
影响因子：
4
作者：
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通讯作者：
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影响因子：
25
作者：
Jia, Yichang;Zhou, Jian;Wang, Yizheng
通讯作者：
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