Phylogeny of Wolbachia pipientis based on gltA, groEL and ftsZ gene sequences:: clustering of arthropod and nematode symbionts in the F supergroup, and evidence for further diversity in the Wolbachia tree
Phylogeny of Wolbachia pipientis based on gltA, groEL and ftsZ gene sequences:: clustering of arthropod and nematode symbionts in the F supergroup, and evidence for further diversity in the Wolbachia tree
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DOI：
10.1099/mic.0.28313-0
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发表时间：
2005-12-01
期刊：
影响因子：
2.8
通讯作者：
Bandi, C
中科院分区：
文献类型：
--
作者：
Casiraghi, M;Bordenstein, SR;Bandi, C
目前属于沃尔巴克氏体属的细胞内细菌的系统发育确定了六个主要分支（A-F），称为“超级群”，但这些超级群的分支顺序仍未得到解决。超群A、B和E包括迄今为止在节肢动物中发现的大多数沃尔巴克氏体，而超群C和D包括在丝虫线虫中发现的大多数沃尔巴克氏体。超群F的成员已在节肢动物（即白蚁）中发现，并且先前已在人类丝虫病的病原体奥氏曼森氏线虫中检测到。为了解决Wolbachia与Mansonella spp.的系统发育位置，以及来自蚤Ctenocephalides felis和丝状线虫Dipetalonema gracile的其他新菌株，作者产生了编码柠檬酸合酶（gltA）、热休克蛋白60（groEL）和细胞分裂蛋白ftsZ的沃尔巴克氏体基因的新DNA序列。系统发育分析证实了Wolbachia从Mansonella spp。作为F超群的一员此外，它被发现，分歧的血统，从迪普。gracile和Cte.猫缺乏任何明确的联系与已知的超群，表明进一步的遗传多样性内沃尔巴克氏体属。最后，虽然生成的数据不允许明确的分辨率的根的全球沃尔巴克氏体树，结果表明，转移沃尔巴克氏体属。从节肢动物到线虫（或反之亦然）可能发生不止一次。
Current phylogenies of the intracellular bacteria belonging to the genus Wolbachia identify six major clades (A-F), termed 'supergroups', but the branching order of these supergroups remains unresolved. Supergroups A, B and E include most of the wolbachiae found thus far in arthropods, while supergroups C and D include most of those found in filarial nematodes. Members of supergroup F have been found in arthropods (i.e. termites), and have previously been detected in the nematode Mansonella ozzardi, a causative agent of human filariasis. To resolve the phylogenetic positions of Wolbachia from Mansonella spp., and other novel strains from the flea Ctenocephalides felis and the filarial nematode Dipetalonema gracile, the authors generated new DNA sequences of the Wolbachia genes encoding citrate synthase (gltA), heat-shock protein 60 (groEL), and the cell division protein ftsZ. Phylogenetic analysis confirmed the designation of Wolbachia from Mansonella spp. as a member of the F supergroup. In addition, it was found that divergent lineages from Dip. gracile and Cte. felis lack any clear affiliation with known supergroups, indicating further genetic diversity within the Wolbachia genus. Finally, although the data generated did not permit clear resolution of the root of the global Wolbachia tree, the results suggest that the transfer of Wolbachia spp. from arthropods to nematodes (or vice versa) probably occurred more than once.